Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer

文献类型： 外文期刊

第一作者： Yao, Ya-Xin

作者： Yao, Ya-Xin;Li, He-Ping;Yao, Ya-Xin;Guan, Wei-Jun;Li, Xiang-Chen;Su, Xiao-Hua;Ma, Yue-Hui

作者机构：

关键词： TSA;histone acetylation;donor cell;reprogramming;IVF;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 11 期

页码：

收录情况： SCI

摘要： An increased histone acetylation is associated with more effective formation of DNA replication complexes (Trichostatin A, TSA), a histone deacetylase inhibitor is able to enhance the pool of acetylated histone. Researchers observed the changes of the Sheep Fibroblasts Cells (SFCs) and Sheep Cumulus Cells (SCCs) being treated with TSA, a stronger immunofluorescent signal for acH4K12 was detected in the 1 cell stage (p<0.05) also the developmental rates of cloned embryos was increased significantly (p<0.05) however, a higher acetylation level were observed in SCCs naturely, no significant effects on acetylation level and developmental rates of cloned embryos were detected after TSA treatment. Moreover, the acetylation distribution in the group of SFC-TSA cloned embryos resembles that in In Vitro Fertilisation (IVF) embryos but T SA had no ability to modify the acetylation state of SCCs cloned embryos similar with the IVF embryos, only a hyperacetylate pattern was detected which means a proper pattern of acetylation In. cloned embryo is more prone to be reprogrammed than hyperacetylation.

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