Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry

文献类型： 外文期刊

第一作者： Li Shan-shan

作者： Li Shan-shan;Wang Jia-qi;Wei Hong-yang;Yang Yong-xin;Bu Deng-pan;Zhang Le-ying;Zhou Ling-yun

作者机构：

关键词： bovine milk casein;phosphorylation;mass spectrometry;titanium dioxide enrichment

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Protein phosphorylation is an important post-translational modification that regulates milk protein structure and function. The objective of this study was to analyze the presence of phosphorylated casein. Bovine milk proteins were first separated by SDS polyacrylamide gel electrophoresis. After in gels digestion and extraction, phosphorylated peptides were enriched by titanium dioxide and identified by ultra performance liquid chromatography coupled with nano electrospray ionization tandem mass spectrometry. This method ensured the identification of 20 phosphorylated peptides, including 7 phosphorylated forms of alpha(s1)-casein, 8 alpha(s2)-casein, and 5 beta-casein. Eight phosphorylated sites derived from 3 alpha(s1)-caseins, 3 alpha(s2)-caseins, and 2 beta-caseins were also identified, and localized on residues Ser(61), Ser(63) and Ser(130) in alpha(s1)-casein; Thr(145), Ser(146) and Ser(158) in alpha(s2)-casein; and Ser(50) and Thr(56) in beta-casein. These findings provide valuable information for investigating casein phosphorylation of the bovine milk.

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