Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs

文献类型： 外文期刊

第一作者： Yi, Li

作者： Yi, Li;Cheng, Shipeng;Xu, Hongli;Wang, Jianke;Cheng, Yuening;Yang, Shen;Luo, Bin

作者机构：

关键词： Canine distemper virus;Differential diagnosis;Phylogenetic analysis

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2012 年 179 卷 1 期

页码：

收录情况： SCI

摘要： A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335 bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555 bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and differentiated the CDV wild-type and vaccine strains by two separate RT-PCRs. The method can be used for clinical detection and epidemiological surveillance. The phylogenetic analysis of the hemagglutinin gene of the local wild-type CDV strains revealed that the seven local isolates all belonged to the Asia-1 lineage, and were clustered closely with one another at the same location. These results suggested that the CDV genotype Asia-1 is circulating currently in domestic dogs in China. (C) 2011 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Phylogenetic analysis of canine distemper virus in domestic dogs in Nanjing, China. Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia.

[2]A multiplex nested RT-PCR for the detection and differentiation of wild-type viruses from C-strain vaccine of classical swine fever virus. Li, Yan,Zhao, Jian-Jun,Li, Na,Shi, Zixue,Cheng, Dan,Zhu, Qing-Hu,Tu, Changchun,Tong, Guang-Zhi,Qiu, Hua-Ji. 2007

[3]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[4]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[5]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[6]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

[7]Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Liao, Yonghong,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Zhang, Tao,Wen, Guoyuan,Hu, Zhibin,Song, Nianhua,Zhang, Tao. 2011

[8]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

[9]Characterization of a new dog isolate of canine distemper virus from China. Qiao, J.,Meng, Q.,Chen, C.,Xia, X.,Ren, Y.,Zhang, H.,Qiao, J.,Cai, X.. 2011

[10]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[11]Recombinant Newcastle disease viral vector expressing hemagglutinin or fusion of canine distemper virus is safe and immunogenic in minks. Ge, Jinying,Wang, Xijun,Tian, Meijie,Wen, Zhiyuan,Yu, Guimei,Zhou, Weiwei,Zu, Shulong,Bu, Zhigao,Gao, Yuwei.

[12]Identification of a novel canine distemper virus B-cell epitope using a monoclonal antibody against nucleocapsid protein. Yi, Li,Cheng, Yuening,Zhang, Miao,Cao, Zhigang,Tong, Mingwei,Wang, Jianke,Zhao, Hang,Lin, Peng,Cheng, Shipeng.

[13]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[14]Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China. Cheng, Yuening,Wang, Jianke,Zhang, Miao,Zhao, Jianjun,Shao, Xiqun,Zhao, Hang,Lin, Peng,Wu, Hua,Ma, Zengjun.

[15]Pathogenesis of canine distemper virus in experimentally infected raccoon dogs, foxes, and minks. Zhao, Jianjun,Shi, Ning,Sun, Yangang,Zhu, Chunsheng,Zhang, Hailing,Hu, Bo,Bai, Xue,Yan, Xijun,Zhao, Jianjun,Yan, Xijun,Martella, Vito,Nikolin, Veljko.

[16]Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates. Yi, Li,Cao, Zhigang,Tong, Mingwei,Cheng, Yuening,Li, Shuang,Wang, Jianke,Lin, Peng,Sun, Yaru,Zhang, Miao,Cheng, Shipeng,Yang, Yong. 2017

[17]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[18]Emergence of canine distemper virus strains with two amino acid substitutions in the haemagglutinin protein, detected from vaccinated carnivores in North-Eastern China in 2012-2013. Zhao, Jianjun,Zhang, Hailing,Bai, Xue,Hu, Bo,Sun, Yangang,Zhu, Chunsheng,Zhang, Lei,Liu, Hao,Xu, Shujuan,Shao, Xiqun,Wu, Wei,Yan, Xijun,Zhao, Jianjun,Yan, Xijun,Martella, Vito. 2014

[19]Development and characterization of neutralizing monoclonal antibodies against canine distemper virus hemagglutinin protein. Bi, Zhenwei,Xia, Xingxia,Wang, Yongshan,Mei, Yongjie,Bi, Zhenwei,Xia, Xingxia,Wang, Yongshan,Mei, Yongjie.

[20]Development of CDV-specific monoclonal antibodies for differentiation of variable epitopes of nucleocapsid protein. Bi, Zhenwei,Wang, Yongshan,Pan, Qunxing,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Pan, Qunxing,Xia, Xingxia,Xu, Libo.

作者其他论文 更多>>

微信小程序