Development of a polyprobe for the simultaneous detection of four grapevine viroids in grapevine plants

文献类型： 外文期刊

第一作者： Peng, Shan

作者： Peng, Shan;Jiang, Dongmei;Pan, Song;Li, Shifang;Jiang, Dongmei;Wang, Hongqing

作者机构：

关键词： Detection;Dot blot hybridization;Grapevine viroid;Polyprobe

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2012 年 132 卷 1 期

页码：

收录情况： SCI

摘要： This study aimed to develop a polyprobe for the simultaneous detection of four viroids that infect grapevine: Hop stunt viroid (HSVd), Australian grapevine viroid (AGVd), Grapevine yellow speckle viroid-1 and 2 (GYSVd-1, 2), using a non-isotopic dot blot hybridization technique. A polyprobe was constructed by cloning tandem full-length sequences of HSVd, AGVd and GYSVd-1 into a single vector. The cRNA polyprobe detected all four viroids with similar sensitivity to that obtained using individual probes. In addition, samples of 78 varieties from Beijing and Xinjiang were analyzed using the polyprobe to survey the incidence of grapevine viroids in China. The result demonstrated that grapevine viroids were detected in 56 (71.8%) varieties. In this study, a rapid, reliable and cost-effective approach to the simultaneous detection of four grapevine viroids has been developed which has the potential for routine use in quarantine and certification programs.

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