Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

文献类型： 外文期刊

第一作者： Chen Shuai

作者： Chen Shuai;Liu Guan-shan;Wang Yuan-ying;Sun Yu-he;Chen Jia

作者机构： Chinese Acad Agr Sci, Key Lab Genet Improvement & Biotechnol, Tobacco Res Inst, Qingdao 166101, Peoples R China;China Agr Univ, State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Beijing 100193, Peoples R China

关键词： abiotic stress;CDPK;Nicotiana tabacum;RACE;real-time qRT-PCR

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 2013影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca(2+) signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.

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