Isolation and expression analysis of NtCHS6, a new chalcone synthase gene from Nicotiana tabacum

文献类型： 外文期刊

第一作者： Chen Shuai

作者： Chen Shuai;Cao Mo-ju;Pan Guang-tang;Chen Shuai;Zhang Yin-chao;Pan Xu-hao;Li Yi-ting;Cui Li-jie;Wu Feng-yan;Yang Ai-guo

作者机构： Sichuan Agr Univ, Maize Res Inst, Chengdu 611130, Peoples R China;Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China

关键词： chalcone synthase;Nicotiana tabacum;leaf-specific expression;light responsiveness;drought responsiveness

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 2020影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 7 期

页码：

收录情况： SCI

摘要： Chalcone synthases (CHS, EC 2.3.1.74) are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synthase shared high homology with the NSCHSL (Y14507) gene and contained most of the conserved active sites that are in CHS proteins. The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum. The expression analysis indicated that NtCHS6 was abundantly expressed in leaves, especially in mature leaves. By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected. Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress. Our results suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and might play important roles in ultraviolet protection and drought tolerance.

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