NUCLEAR AND CHLOROPLAST SSR MARKERS IN PAEONIA DELAVAYI (PAEONIACEAE) AND CROSS-SPECIES AMPLIFICATION IN P. LUDLOWII
文献类型: 外文期刊
第一作者: Zhang, Jin-Mei
作者: Zhang, Jin-Mei;Liu, Jing;Sun, Hai-Lu;Yu, Jing;Wang, Jian-Xiu;Zhou, Shi-Liang;Zhang, Jin-Mei;Zhang, Jin-Mei;Liu, Jing;Sun, Hai-Lu;Yu, Jing;Wang, Jian-Xiu
作者机构:
关键词: genetic diversity;microsatellite marker;Paeonia delavayi;Paeonia ludlowii;Paeoniaceae;population genetic structure
期刊名称:AMERICAN JOURNAL OF BOTANY ( 影响因子:3.844; 五年影响因子:3.657 )
ISSN: 0002-9122
年卷期: 2011 年 98 卷 12 期
页码:
收录情况: SCI
摘要: Premise of the study: Microsatellite primers were developed for Paeonia delavayi and P. ludlowii (Paeoniaceae) to study their population genetics and phytogeography. Methods and Results: Nine polymorphic nuclear microsatellite loci were isolated from an enriched library of P. delavayi and primers were designed. The number of alleles per locus ranged from two to 16; the observed and expected heterozygosities ranged from 0.014 to 0.687 and 0.042 to 0.875, respectively. Six polymorphic chloroplast microsatellite loci were identified in P. delavayi and primers were provided. The number of alleles per locus ranged from two to six and the polymorphic information content ranged from 0.08 to 0.716. Both nuclear and chloroplast primers were successfully applicable to P. ludlowii. Conclusions: The markers developed here will facilitate analyses of genetic diversity, population genetic structure, phytogeographical patterns, and conservation for P. delavayi and P. ludlowii
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