Manganese Regulates Manganese-Containing Superoxide Dismutase (MnSOD) Expression in the Primary Broiler Myocardial Cells

文献类型： 外文期刊

第一作者： Gao, Tianquan

作者： Gao, Tianquan;Zhang, Keying;Li, Sufen;Luo, Xugang;Li, Sufen;Luo, Xugang;Wang, Funing;Li, Sufen

作者机构：

关键词： MnSOD;Manganese;Myocardial cell;Gene expression regulation

期刊名称：BIOLOGICAL TRACE ELEMENT RESEARCH （ 影响因子：3.738； 五年影响因子：3.44 ）

ISSN： 0163-4984

年卷期： 2011 年 144 卷 1-3 期

页码：

收录情况： SCI

摘要： Previous studies showed that dietary manganese can increase the MnSOD mRNA expression in a dose-dependent manner in the heart of broilers. In order to explore the specific mechanism of the MnSOD expression induced by manganese, a model of MnSOD expression was developed with primary cultured broiler myocardial cells. The objective of the present study was to investigate whether the model was working or not and to determine how manganese affects the expression of the enzyme in broiler myocardial cells in vitro. In experiment 1, various amount of manganese (0, 0.25, 0.5, 1, 2, and 4 mM) were added into the cultures for 24-h incubation to investigate MnSOD expression and for 0-, 6-, 12-, 24-, 36-, and 48-h incubation to measure the cell viability. In experiment 2, the most suitable Mn supplementation based on the results of experiment 1 was added into cultures for 6-, 12-, 24-, and 48-h incubation. The results showed that MnSOD mRNA, MnSOD protein, and MnSOD activity were induced by manganese in dose- and time-dependent manner. Manganese regulates MnSOD expression not only at transcriptional level but also at translational and/or posttranslational levels.

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