Characterization of a laccase gene from the white-rot fungi Trametes sp 5930 isolated from Shennongjia Nature Reserve in China and studying on the capability of decolorization of different synthetic dyes

文献类型： 外文期刊

第一作者： Yang, Yang

作者： Yang, Yang;Ma, Fuying;Yu, Hongbo;Fan, Fangfang;Zhang, Xiaoyu;Yang, Yang;Wan, Xia;Jiang, Mulan

作者机构：

关键词： White-rot fungi;Laccase gene;Decolorization of dyes;Enzymes;DNA;Yeast

期刊名称：BIOCHEMICAL ENGINEERING JOURNAL （ 影响因子：3.978； 五年影响因子：4.084 ）

ISSN： 1369-703X

年卷期： 2011 年 57 卷

页码：

收录情况： SCI

摘要： Laccase belongs to a family of multi-copper oxidases which is especially useful for biotechnological and industrial applications. A laccase-producing white-rot fungi strain designated as Trametes sp. 5930 was nearly isolated from Shennongjia Nature Reserve in China. Trametes sp. 5930 had the high yield of laccase and was capable of clecolorizing different dyes efficiently. Laccase played a very important role in the decolorization of different dyes by this fungus. The laccase gene lac5930-1 and its corresponding full-length cDNA were then cloned and characterized from Trametes sp. 5930. The 1563 bp full-length cDNA of lac5930-1 encoded a mature laccase protein consisting of 499 amino acids preceded by a signal peptide of 21 amino acids. lac5930-1 gene was successfully expressed in Pichia pastoris, which verified the function of lac5930-1 encoding active laccase by means of gene expression. The recombinant laccase produced by the yeast transformant in which lac5930-1 was efficiently expressed, conferred the ability to decolorize different dyes. The capability of decolorizing different dyes was positively related to the laccase activity, which provided strong evidence for the important function of laccase used in decolorizing industrial dyes. (C) 2011 Published by Elsevier B.V.

分类号：

  • 相关文献

[1]Decolorization of different dyes by a newly isolated white-rot fungi strain Ganoderma sp.En3 and cloning and functional analysis of its laccase gene. Zhuo, Rui,Ma, Li,Fan, Fangfang,Zhang, Xiaoyu,Yang, Yang,Zhuo, Rui,Fan, Fangfang,Gong, Yangmin,Wan, Xia,Jiang, Mulan,Yang, Yang. 2011

[2]Cloning and functional analysis of a new laccase gene from Trametes sp 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. Fan, Fangfang,Zhuo, Rui,Sun, Su,Zhang, Xiaoyu,Yang, Yang,Fan, Fangfang,Zhuo, Rui,Wan, Xia,Jiang, Mulan,Yang, Yang. 2011

[3]Characterization of a yeast recombinant laccase rLAC-EN3-1 and its application in decolorizing synthetic dye with the coexistence of metal ions and organic solvents. Zhuo, Rui,He, Feng,Zhang, Xiaoyu,Yang, Yang,Zhuo, Rui,Yang, Yang.

[4]Structure-based fragment shuffling of two fungal phytases for combination of desirable properties. Wang, Xunzhang,Bei, Jinlong,Chen, Zhuang,Jiang, Zongyong.

[5]SYMBIOTIC CULTURE OF WHITE-ROT FUNGI AND AEROBIC DENITRIFIER BACTERIA IMMOBILIZED BY BACTERIAL CELLULOSE TO TREAT MIXED WASTEWATER COMPOSED OF DYE AND DOMESTIC SEWAGE. Qiao, Nan,Liu, Yang,Yang, Meng-yu,Gao, Bo,Zhang, Jin-bang.

[6]The genome of Pleurotus eryngii provides insights into the mechanisms of wood decay. Bao, Da-Peng.

[7]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[8]Unique sequence characteristics of genes in the leftmost region of unique long region in duck enteritis virus. Liu, Xiaoli,Liu, Shengwang,Li, Huixin,Han, Zongxi,Shao, Yuhao,Kong, Xiangang.

[9]Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Chu, M. X.,Jiao, C. L.,He, Y. Q.,Wang, J. Y.,Liu, Z. H.,Chen, G. H..

[10]Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.. Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Wang, Yun,Lou, Kai,Lee, Jae-Chan,Kim, Chang-Jin.

[11]Identification and Characterization of a Glyoxalase I Gene in a Rapeseed Cultivar with Seed Thermotolerance. Yan, Guixin,Lv, Xiaodan,Gao, Guizhen,Li, Feng,Li, Jun,Qiao, Jiangwei,Xu, Kun,Chen, Biyun,Wang, Limin,Xiao, Xin,Wu, Xiaoming. 2016

[12]Microbial Diversity and Biochemical Analysis of Suanzhou: A Traditional Chinese Fermented Cereal Gruel. Qin, Huibin,Sun, Qinghui,Pan, Xuewei,Yang, Hongjiang,Qin, Huibin,Qiao, Zhijun. 2016

[13]Effects of different forms of yeast Saccharomyces cerevisiae on growth performance, intestinal development, and systemic immunity in early-weaned piglets. Jiang, Zongyong,Wei, Shaoyong,Wang, Zhilin,Hu, Shenglan,Zheng, Chuntian,Hu, Youjun,Wang, Li,Ma, Xianyong,Yang, Xuefen,Jiang, Zongyong,Zhu, Cui,Chen, Zhuang. 2015

[14]Brassica napus soluble epoxide hydrolase (BNSEH1) - Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris. Bellevik, S,Zhang, JM,Meijer, J. 2002

[15]Attraction of Coffee Bean Weevil, Araecerus fasciculatus, to Volatiles from the Industrial Yeast Kluyveromyces lactis. Mei, Xiang-Dong,She, Dongmei,Ning, Jun,Zhang, Xiao-Fang,Li, Yao-Fa,Zhang, Tao.

[16]High level expression of a recombinant acid phytase gene in Pichia pastoris. Xiong, AS,Yao, QH,Peng, RH,Han, PL,Cheng, ZM,Li, Y.

[17]Du1, encoding a novel Prp1 protein, regulates starch biosynthesis through affecting the splicing of Wx(b) supercript stop pre-mRNAs in rice (Oryza sativa L.). Zeng, Dali,Yan, Meixian,Wang, Yonghong,Liu, Xinfang,Qian, Qian,Li, Jiayang.

[18]Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum. Gong, Yangmin,Zhang, Junping,Guo, Xiaojing,Wan, Xia,Liang, Zhuo,Hu, Chuan Jiong,Jiang, Mulan,Zhang, Junping.

[19]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. Yang, QC,Wu, MS,Wang, PQ,Kang, JM,Zhou, XL.

[20]Production and Characteristics of High Quality Vinegar from Sugarcane Juice. Lin, Bo,Li, Yang-Rui,Zheng, Feng-Jin,Sun, Jian,Li, Zhi-Chun. 2015

作者其他论文 更多>>

微信小程序