Characteristic analysis of heat-shock-cognate 70-4 gene promoter from silkworm, Bombyx mori (Bmhsc70-4)

文献类型： 外文期刊

第一作者： Tang, Shun-ming

作者： Tang, Shun-ming;Qui, Zhiyong;Zhao, Qiao-ling;Yi, Yong-zhu;Zhang, Zhi-fang

作者机构：

关键词： Bombyx mori;heat shock cognate 70-4;promoter;heat shock;foreign insect hormone;transient expression;in vitro/in vivo

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 68 期

页码：

收录情况： SCI

摘要： Heat shock cognate proteins (HSCs) are homologous to heat shock proteins (HSPs) and constitutively expressed. HSCs facilitate correct protein folding and translocation and could be influenced by a variety of extracellular signals. The 1.4 kb long hsc70-4 promoter from Bombyx mori (Bmhsc70-4) was amplified and cloned into the plasmid for driving the luciferase reporter gene. Transient expression assays were carried out to investigate the effects of heat shock treatment, developmental stages, insect ecdysone and juvenile hormone analogue (JHA) on the Bmhsc70-4 promoter activity. The results demonstrate that the Bmhsc70-4 promoter activity was high in BmN cells, Sf21 cells and silkworm larvae at normal temperature. By heat-shock treatment at 37 degrees for 2 h, the Bmhsc70-4 promoter activity increased by 4.40-, 16.12-, 3.11-folds to the controls in BmN cells, Sf21 cells (Spodoptera frugiperda) and 4th instar silkworm larvae, respectively. But heat-shock treatment for 5th instar silkworm larvae led to a drastically decrease of the activity. Transcriptional activity of promoter was changed with the different developmental stages and reached the highest level at the wandering stage in 5th instar. Ecdysone treatment had no significant effect, while JHA treatments showed typical dose-dependent effects on the Bmhsc70-4 promoter activity in BmN cells. Low level of JHA (1 mu g/ml) increased the Bmhsc70-4 promoter activity, but high level (>5 mu g/ml) decreased its activity. In 5th instar silkworm larvae, spreading of high level JHA (>150 mg/kg) or injection of low level of ecdysone (1 to 3 mu g per os) increased the Bmhsc70-4 promoter activity. From the above results obtained from in vitro and in vivo, we can deduce that the Bmhsc70-4 promoter is both constitutive and inducible promoter. Due to its high transcriptional activity and inducible by the environmental signals, this implies that Bmhsc70-4 promoter is an ideal candidate in application of transgene or cell stable expression system in Bombyx mori.

分类号：

  • 相关文献

[1]Functional analysis of the larval serum protein gene promoter from silkworm, Bombyx mori.. Tang, SM,Yi, YZ,Shen, XJ,Zhang, ZF,Li, YR,He, JL. 2003

[2]Transcriptional regulation of the gene for prothoracicotropic hormone in the silkworm, Bombyx mori. Wei, Zhao-Jun,Yu, Miao,Hong, Gui-Yun,Jiang, Shao-Tong,Tang, Shun-Ming,Yi, Yong-Zhu.

[3]Identification of ecdysone response elements (EcREs) in the Bombyx mori cathepsin D promoter. Yu, Jie,Wu, Feng-Yao,Zou, Feng-Ming,Jia, Jun-Qiang,Wang, Sheng-Peng,Zhang, Guo-Zheng,Guo, Xi-Jie,Gui, Zhong-Zheng,Jia, Jun-Qiang,Wang, Sheng-Peng,Zhang, Guo-Zheng,Guo, Xi-Jie,Gui, Zhong-Zheng.

[4]GENOMIC ANALYSIS OF SILKWORM microRNA PROMOTERS AND CLUSTERS. Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Huang, Yong,Shen, Xing Jia,Huang, Jin Shan,Tang, Shun Ming,Zou, Quan.

[5]Relationship between Omega as well as the length of 3 ' poly (dA) and efficiency of gene expression. Mao, LQ,Guo, SD. 1998

[6]Identification of functional region of helicase gene promoter in Bombyx mori nuclear polyhedrosis virus. Xiao, QL,Zhang, ZF,Yi, YZ,He, JL,Wu, XF. 2002

[7]Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX.

[8]Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine. Wang, Huiling,Wang, Wei,Li, Hui,Zhang, Ping,Zhan, Jicheng,Huang, Weidong,Wang, Wei.

[9]Promoter analysis of Bombyx mori nucleopolyhedrovirus Ubiquitin gene. Lin, Xu'ai,Chen, Yin,Yi, Yongzhu,Zhang, Zhifang,Lin, Xu'ai,Yan, Jie,Chen, Yin.

[10]Characterization of the Autographa californica Nucleopolyhedrovirus Ubiquitin Gene Promoter. Lin, Xu Ai,Chen, Yin,Yi, Yong Zhu,Zhang, Zhi Fang,Xu, Wei Hua.

[11]Construction of Rice Site-Specific Chloroplast Transformation Vector and Transient Expression of EGFP Gene in Dunaliella Salina. Han, Xiaoxia,Zuo, Jia,Xie, Lingling,He, Ronghua,Gao, Jing,Cao, Mengliang,Yuan, Longping,Cao, Mengliang,Chang, Lan,Cao, Mengliang.

[12]Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum. Zhang, HB,Zhang, DB,Chen, J,Yang, YD,Huang, ZJ,Huang, DF,Wang, XC,Huang, RF. 2004

[13]Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1. Gao, Ying,Zan, Xin-Li,Wu, Xue-Feng,Jia, Shuang-Wei,Zhao, Kai-Jun,Zan, Xin-Li,Chen, Yu-Ling,Yao, Lei. 2014

[14]Expression of zma-miR169 miRNAs and their target ZmNF-YA genes in response to abiotic stress in maize leaves. Luan, Mingda,Xu, Miaoyun,Zhang, Lan,Fan, Yunliu,Wang, Lei,Lu, Yunming.

[15]Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus. Xiao, QL,Zhang, ZF,Yi, YZ,He, JL,Wu, XF. 2001

[16]Transient Expression of Exogenous Gene into Plant Cell Mediated by PEI Nanovector. Li Ying,Cui Hai-xin,Song Yu,Li Yao,Huang Jin-li. 2011

[17]An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes. Wang, Huiling,Xu, Haiying,Wang, Huiling,Zhan, Jicheng,Huang, Weidong,Xu, Haiying,Wang, Wei.

[18]Heat stress in wheat (Triticum aestivum L.): Effects on grain growth and quality traits. Spiertz, J. H. J.,Hamer, R. J.,Xu, H.,Primo-Martin, C.,Don, C.,van der Putten, P. E. L.. 2006

[19]HEAT SHOCK INDUCED MORPHO-PHYSIOLOGICAL RESPONSE IN INDICA RICE (ORYZA SATIVA L.) AT EARLY SEEDLING STAGE. Zafar, Syed Adeel,Khan, Abdus Salam,Hameed, Amjad,Ashraf, Muhammad,Zafar, Syed Adeel. 2017

[20]Phosphoinositide-specific phospholipase C9 is involved in the thermotolerance of Arabidopsis. Zheng, Shu-Zhi,Liu, Yu-Liang,Li, Bing,Shang, Zhong-lin,Sun, Da-Ye,Zhou, Ren-Gang. 2012

作者其他论文 更多>>

微信小程序