Nanos2 is a molecular marker of inchoate buffalo spermatogonia

文献类型： 外文期刊

第一作者： Li, Meng-Qi

作者： Li, Meng-Qi;Luo, Ao-Lin;Zhao, Peng-Wei;Li, Ting-Ting;Geng, Shuang-Shuang;Liang, Xing-Wei;Xu, Hui-Yan;Lu, Yang-Qing;Lu, Sheng-Sheng;Yang, Xiao-Gan;Lu, Ke-Huan;Li, Meng-Qi;Luo, Ao-Lin;Zhao, Peng-Wei;Li, Ting-Ting;Geng, Shuang-Shuang;Liang, Xing-Wei;Xu, Hui-Yan;Lu, Yang-Qing;Lu, Sheng-Sheng;Yang, Xiao-Gan;Lu, Ke-Huan;Li, Meng-Qi

作者机构：

关键词： Buffalo;Inchoate spermatogonia;Nanos2;Molecular marker

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2017 年 186 卷

页码：

收录情况： SCI

摘要： Nanos2 belongs to the Nanos gene-coding family and is an important RNA-binding protein that has been shown to have essential roles in male germline stem cells development and self-renewal in mouse. However, little is known about Nanos2 in inchoate buffalo spermatogonia. Here, rapid amplification of cDNA ends (RACE) was used to obtain the full-length buffalo Nanos2 sequence and bioinfonnatic analysis revealed a highly conserved Nanos2 sequence between buffalo and other mammalian species. Although Nanos2 was expressed in various tissues, the highest mRNA expression levels were found in testes tissue. Moreover, Nanos2 mRNA was abundant in fetal and pre-puberal testes but markedly decreased in the testes of adults. At the protein level, immunohistochemistry in pre-puberal testes revealed a pattern of NANOS2 expression similar to that for the undifferentiated type A spermatogonia marker PGP9.5. Furthermore, NANOS2 expression was low in adult testes and restricted to elongating spermatids. Altogether, our data suggest that Nanos2 is a potential preliminary molecular marker of inchoate buffalo spermatogonia, and may play an important role in buffalo spermatogonial stem cells (SSCs) development and self-renewal, as has been observed in other model animals.

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