Molecular processes underlying the ROS-induced changes in muscle hardness in grass carp: Insights from a dietary pro-oxidant and oxidant approach

文献类型： 外文期刊

第一作者： Yu, Ermeng

作者： Yu, Ermeng;Chen, Lunjian;Wang, Guangjun;Xie, Jun;Tian, Jingjing;Li, Hongyan;Xia, Yun;Zhang, Kai;Li, Zhifei;Gong, Wangbao;Kaneko, Gen

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关键词： Ctenopharyngodon idellus; muscle hardness; pro-oxidants; reactive oxygen species

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.184； 五年影响因子：2.447 ）

ISSN： 1355-557X

年卷期： 2022 年 53 卷 17 期

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收录情况： SCI

摘要： Reactive oxygen species (ROS) is known to increase muscle hardness, which has been of particular interest in food animals, but the detailed mechanism for the ROS-induced changes remains unclear. To explore the responsible molecular pathway, we conducted a detailed gene/protein expression analysis on the crisp grass carp model using four groups fed with different diets: control commercial feed (C group), a natural pro-oxidant faba bean (FB group) only, FB + antioxidants vitamins C and E (FB + AO group), and commercial feed + a pro-oxidant vitamin K3 (O group). Real-time PCR and parallel reaction monitoring, an emerging high-resolution mass spectrometry analysis, were employed. Our expression analysis highlighted two main effects of dietary pro-oxidants: endogenous ROS production and enhancement of muscle regeneration. The CGC and O groups generally increased the expression of genes/proteins related to ROS generation (e.g. mitochondrial proteins), caspase 3 (a candidate mediator of muscle regeneration) and muscle structural proteins such as tropomyosin and collagen. Most changes were not observed in the FB + AO group, indicating the causal role of ROS in the observed changes. These results explain part of molecular processes of ROS-induced improvement in muscle hardness in grass carp and would be useful for further application of dietary pro-oxidants to other species.

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