Doxycycline enhances adsorption and inhibits early-stage replication of porcine reproductive and respiratory syndrome virus in vitro

文献类型： 外文期刊

第一作者： Li, Yuming

作者： Li, Yuming;Wu, Zhuanchang;Liu, Ke;Qi, Pengfei;Xu, Jinpeng;Wei, Jianchao;Li, Beibei;Shao, Donghua;Shi, Yuanyuan;Qiu, Yafeng;Ma, Zhiyong

作者机构：

关键词： doxycycline;porcine reproductive and respiratory syndrome virus;antiviral activity;adsorption;antiviral drug

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2017 年 364 卷 17 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has an important impact on the pig industry. Doxycycline (Dox) is a second-generation tetracycline widely used for treating bacterial infections. We evaluated the antiviral effect of Dox against PRRSV infection in Marc-145 cells that were susceptible to PRRSV infection. Dox significantly reduced the PRRSV-induced cytopathic effect and effectively restrained PRRSV replication in a dose-dependent manner. The 50% effective concentration of Dox was approximately 0.25 +/- 0.05 mu g/ml. We also determined the stage at which Dox influenced PRRSV replication, and showed that Dox enhanced PRRSV adsorption and inhibited the early stage of PRRSV replication after viral entry into host cells. These observations demonstrate that Dox is able to restrain PRRSV infection in cultured cells.

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