Molecular and biochemical characterization of a new alkaline beta-propeller phytase from the insect symbiotic bacterium Janthinobacterium sp TN115

文献类型： 外文期刊

第一作者： Zhang, Rui

作者： Zhang, Rui;Yang, Peilong;Huang, Huoqing;Yuan, Tiezheng;Shi, Pengjun;Meng, Kun;Yao, Bin

作者机构：

关键词： beta-Propeller phytase;Janthinobacterium sp TN115;Alkaline phytase;Batocera horsfieldi (Coleoptera: Cerambycidae)

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2011 年 92 卷 2 期

页码：

收录情况： SCI

摘要： A phytase-encoding gene (phyA115) was cloned from Janthinobacterium sp. TN115, a symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae (Coleoptera: Cerambycidae), and expressed in Escherichia coli. The 1,884-bp full-length gene encodes a 28-residue putative signal peptide and a 599-residue mature protein with a calculated mass of 64 kDa. The deduced PhyA115 shares low identity with known sequences (47% at most) and contains an N-terminal incomplete domain (residues 29-297; domain N) and a typical beta-propeller phytase domain at the C terminus (residues 298-627; domain C). Distinct from other beta-propeller phytases that have neutral pH optima (pH 6.0-7.5), purified recombinant PhyA115 exhibits maximal activity at pH 8.5 and 45A degrees C in the presence of 1 mM Ca(2+) and is highly active over a wider pH range (pH 6.0-9.0). These results indicate that PhyA115 is a beta-propeller phytase that has application potential in aquaculture feed. To our knowledge, this is the first report of cloning of a phytase gene from the symbiotic microbes of an insect digestive tract and from the genus Janthinobacterium. The N-terminal incomplete domain is found to have no phytase activity but can influence the pH property of PhyA115.

分类号：

  • 相关文献

[1]Two Types of Phytases (Histidine Acid Phytase and beta-Propeller Phytase) in Serratia sp TN49 from the Gut of Batocera horsfieldi (Coleoptera) Larvae. Zhang, Rui,Yang, Peilong,Huang, Huoqing,Shi, Pengjun,Yuan, Tiezheng,Yao, Bin,Zhang, Rui.

作者其他论文 更多>>

微信小程序