Cross-resistance and biochemical mechanisms of abamectin resistance in the western flower thrips, Frankliniella occidentalis

文献类型: 外文期刊

第一作者: Chen, Xuelin

作者: Chen, Xuelin;Yuan, Linze;Du, Yuzhou;Wang, Jianjun;Zhang, Youjun

作者机构:

关键词: Frankliniella occidentalis;Abamectin;Cross-resistance;Resistance mechanism;Cytochrome P450 monooxygenase

期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:3.963; 五年影响因子:4.454 )

ISSN: 0048-3575

年卷期: 2011 年 101 卷 1 期

页码:

收录情况: SCI

摘要: Abamectin resistance was selected in the western flower thrips [Frankliniella occidentalis (Pergande)] under the laboratory conditions, and cross-resistance patterns and possible resistance mechanisms in the abamectin-resistant strain (ABA-R) were investigated. Compared with the susceptible strain (ABA-S), the ABA-R strain displayed 45.5-fold resistance to abamectin after 15 selection cycles during 18 generations. Rapid reversion of abamectin resistance was observed in the ABA-R strain in the absence of the insecticide selection pressure. Moderate and low levels of cross-resistance to chlorpyrifos (RR 11.4) and lambda-cyhalothrin (3.98) were observed in the ABA-R strain, but no significant cross-resistance was found to spinosad (2.00), acetamiprid (1.47) and chlorfenapyr (0.26). Our studies also showed that the esterase inhibitor S,S,S-tributyl phosphorotrithioate (DEF) and glutathione S-transferase inhibitor diethyl maleate (DEM) were not able to synergize the toxicity of abamectin, whereas the oxidase inhibitor piperonyl butoxide (PBO) conferred a significant synergism on abamectin in the ABA-R strain (SR 3.00). Biochemical analysis showed that cytochrome P450 monooxygenase activity of the ABA-R strain was 6.66-fold higher than that of the ABA-S strain. It appears that enhanced oxidative metabolism mediated by cytochrome P450 monooxygenases was a major mechanism for abamectin resistance in the western flower thrips. (C) 2011 Elsevier Inc. All rights reserved.

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