Identification of Soil-Borne Fungi Using Fourier Transform Infrared Spectroscopy

文献类型： 外文期刊

第一作者： Chai A-li

作者： Chai A-li;Li Bao-ju;Shi Yan-xia;Xie Xue-wen

作者机构：

关键词： FTIR;Soil-borne fungi;Discriminant analysis;Cluster analysis;Identification

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2011 年 31 卷 8 期

页码：

收录情况： SCI

摘要： Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme Fusarium semitectum Fusarium oxysporum, Fusarium solani, Rhizoctonia solani Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from which the peak wave numbers showing greatest variability were selected: 2 924, 2 854, 1 745, 1 641, 1547, 1 466, 1 406, 1 376, 1 306, 1 240, 1 201, 1152, 1 109 and 1 028 cm(-1). To discriminate different fungal strains, canonical discriminant analysis and cluster analysis were performed at these characteristic wave numbers. Results showed that the classification accuracies achieved 100% for different species of fungi, and classification accuracies for different fusarium strains achieved 95. 56%, demonstrating the high potential of this technique for fungi identification.

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