Protective Effects of Cynaroside Against H2O2-Induced Apoptosis in H9c2 Cardiomyoblasts

文献类型： 外文期刊

第一作者： Sun, Xiao-bo

作者： Sun, Xiao-bo;Sun, Xiao-bo

作者机构：

关键词： CYNAROSIDE;OXIDATIVE STRESS;APOPTOSIS;MITOCHONDRIAL DYSFUNCTION

期刊名称：JOURNAL OF CELLULAR BIOCHEMISTRY （ 影响因子：4.429； 五年影响因子：4.266 ）

ISSN： 0730-2312

年卷期： 2011 年 112 卷 8 期

页码：

收录情况： SCI

摘要： Flavonoids with potent anti-oxidative effects are the major effective components in traditional herbal medicine used in treating cardiovascular diseases. Cynaroside is a flavonoid compound that exhibits anti-oxidative capabilities. However, little is known about its effect on oxidative injury to cardiac myocytes and the underlying mechanisms. This study was designed to investigate the protective effects of cynaroside against H2O2-induced apoptosis in H9c2 cardiomyoblasts. H9c2 cells were pretreated with cynaroside for 4 h before exposure to 150 mu M H2O2 for 6 h. H2O2 treatment caused severe injury to the H9c2 cells, which was accompanied by apoptosis, as revealed by analysis of cell nuclear morphology, through Annexin V FITC/PI staining and caspase proteases activation. Cynaroside pretreatment significantly reduced the apoptotic rate by enhancing the endogenous anti-oxidative activity of superoxide dismutase, glutathione peroxidase, and catalase, thereby inhibiting intracellular reactive oxygen species (ROS) generation. Moreover, cynaroside moderated H2O2-induced disruption of mitochondrial membrane potential, increased the expression of anti-apoptotic protein Bcl-2 while decreased the expression of proapoptotic protein Bax, and thereby inhibited the release of apoptogenic factors (cytochrome c and smac/Diablo) from mitochondria in H9c2 cells. Our data also demonstrated that cynaroside pretreatment showed an inhibitory effect on the H2O2-induced increase in c-Jun N-terminal kinase (JNK) and P53 protein expression. These results suggest that cynaroside prevents H2O2-induced apoptosis in H9c2 cell by reducing the endogenous production of ROS, maintaining mitochondrial function, and modulating the JNK and P53 pathways. J. Cell. Biochem. 112: 2019-2029, 2011. (C) 2011 Wiley-Liss, Inc.

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