Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-beta
文献类型: 外文期刊
第一作者: Chen, Weiye
作者: Chen, Weiye;Hu, Qianqian;Qu, Linmao;Li, HaoBo;Huang, Kehe;Chen, Weiye;Cao, Wenyan;Zhao, Huijun;Hu, Qianqian;Qu, Linmao;Hu, Sen;Ge, Jinying;Wen, Zhiyuan;Wang, Xijun;Li, HaoBo;Bu, Zhigao
作者机构:
关键词: Porcine interferon-beta;CHO-K1 cells;Stable expression;Biological activity;Antiviral activity
期刊名称:CYTOKINE ( 影响因子:3.861; 五年影响因子:4.257 )
ISSN: 1043-4666
年卷期: 2011 年 54 卷 3 期
页码:
收录情况: SCI
摘要: A CHO cell clone (CHO-PoIFN-beta) with stable porcine IFN-beta expression under control of CMV promoter was selected under G418 pressure. In a 25 cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-beta reached 2.3 x 10(6) IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-beta could induce the expression of porcine Mx protein in PK15 cells, and activate the chicken Mx promoter-controlled luciferase reporter gene expression, confirming that the recombinant PoIFN-beta has the biological activity of natural porcine type-I interferon. In addition, the recombinant PoIFN-beta fully protected PK15 cells against 1000 TCID50 of porcine transmissible gastroenteritis virus and pseudo-rabies virus infection, demonstrating its high potential in therapeutic applications. This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-beta. (C) 2010 Published by Elsevier Ltd.
分类号:
- 相关文献
作者其他论文 更多>>
-
2023 International African Swine Fever Workshop: Critical Issues That Need to Be Addressed for ASF Control
作者:Wang, Lihua;Li, Yuzhen;Shi, Jishu;Ganges, Llilianne;Ganges, Llilianne;Dixon, Linda K.;Netherton, Christopher L.;Reis, Ana L.;Bu, Zhigao;Zhao, Dongming;Quang Lam Truong;Richt, Juergen A.;Jin, Meilin;Peng, Guiqing;Benarafa, Charaf;Summerfield, Artur;Benarafa, Charaf;Summerfield, Artur;Benarafa, Charaf;Summerfield, Artur;Weng, Changjiang;Han, Jun;Penrith, Mary-Louise;Mo, Yupeng;Su, Zhipeng;Dang Vu Hoang;Pogranichniy, Roman M.;Balaban-Oglan, David-Adrian;Balaban-Oglan, David-Adrian;Wang, Kewen;Cai, Xuepeng
关键词:African swine fever; workshop; platform; highlights; lessons; control; prevention
-
Tubeimosides are pan-coronavirus and filovirus inhibitors that can block their fusion protein binding to Niemann-Pick C1
作者:Khan, Ilyas;Li, Sunan;Tao, Lihong;Wang, Chong;Liu, Xiaoyang;Ahmad, Iqbal;Su, Wenqiang;Zhong, Gongxun;Wen, Zhiyuan;Wang, Jinliang;Hua, Rong-Hong;Wan, Xiao-Peng;Bu, Zhi-Gao;Ye, Bowei;Li, Huiyu;Ma, Ao;Liang, Jie;Zheng, Yong-Hui
关键词:
-
African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway
作者:Ye, Guangqiang;Zhang, Zhaoxia;Liu, Xiaohong;Liu, Hongyang;Chen, Weiye;Feng, Chunying;Li, Jiangnan;Zhou, Qiongqiong;Zhao, Dongming;Zhang, Shuai;Chen, Hefeng;Bu, Zhigao;Huang, Li;Weng, Changjiang;Zhang, Zhaoxia;Li, Jiangnan;Huang, Li;Weng, Changjiang
关键词:ASFV; pH240R; ISGs; IFN; JAK-STAT; viral replication
-
Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing
作者:Xie, Yan;Liu, Xianan;Luo, Chaohu;Hu, Qianqian;Che, Xuan;Zhao, Min;Wu, Liqi;Zhao, Liling;Ding, Ming
关键词:Begomovirus; Tomato yellow leaf curl Chuxiong virus; Whitefly; Molecular identity; Symptom determinant; Post-transcriptional gene silencing
-
A highly efficient blocking ELISA based on p72 monoclonal antibody for the detection of African swine fever virus antibodies and identification of its linear B cell epitope
作者:Tesfagaber, Weldu;Wang, Wan;Wang, Lulu;Zhao, Rui;Zhu, Yuanmao;Li, Fang;Sun, Encheng;Liu, Renqiang;Bu, Zhigao;Zhao, Dongming;Zhao, Rui;Zhao, Dongming;Meng, Geng
关键词:African swine fever virus; P72; Monoclonal antibodies; Blocking ELISA; Linear epitope
-
African swine fever virus pB318L, a trans-geranylgeranyl-diphosphate synthase, negatively regulates cGAS-STING and IFNAR-JAK-STAT signaling pathways
作者:Liu, Xiaohong;Chen, Hefeng;Ye, Guangqiang;Liu, Hongyang;Feng, Chunying;Chen, Weiye;Hu, Liang;Zhou, Qiongqiong;Zhang, Zhaoxia;Li, Jiangnan;Zhang, Xianfeng;He, Xijun;Guan, Yuntao;Wu, Zhengshuang;Zhao, Dongming;Bu, Zhigao;Weng, Changjiang;Huang, Li;Zhang, Zhaoxia;Li, Jiangnan;Weng, Changjiang;Huang, Li
关键词:
-
Identification of two novel B cell epitopes on E184L protein of African swine fever virus using monoclonal antibodies
作者:Tesfagaber, Weldu;Wang, Wan;Zhao, Rui;Yin, Li;Zhu, Yuanmao;Sun, Encheng;Liu, Renqiang;Lin, Wenjun;Bu, Zhigao;Li, Fang;Zhao, Dongming;Lan, Desong;Yang, Mingyang;Zhao, Rui;Zhao, Dongming
关键词:African swine fever virus; E184L; Linear B cell epitope; Monoclonal antibody