Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-beta
文献类型: 外文期刊
第一作者: Chen, Weiye
作者: Chen, Weiye;Hu, Qianqian;Qu, Linmao;Li, HaoBo;Huang, Kehe;Chen, Weiye;Cao, Wenyan;Zhao, Huijun;Hu, Qianqian;Qu, Linmao;Hu, Sen;Ge, Jinying;Wen, Zhiyuan;Wang, Xijun;Li, HaoBo;Bu, Zhigao
作者机构:
关键词: Porcine interferon-beta;CHO-K1 cells;Stable expression;Biological activity;Antiviral activity
期刊名称:CYTOKINE ( 影响因子:3.861; 五年影响因子:4.257 )
ISSN: 1043-4666
年卷期: 2011 年 54 卷 3 期
页码:
收录情况: SCI
摘要: A CHO cell clone (CHO-PoIFN-beta) with stable porcine IFN-beta expression under control of CMV promoter was selected under G418 pressure. In a 25 cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-beta reached 2.3 x 10(6) IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-beta could induce the expression of porcine Mx protein in PK15 cells, and activate the chicken Mx promoter-controlled luciferase reporter gene expression, confirming that the recombinant PoIFN-beta has the biological activity of natural porcine type-I interferon. In addition, the recombinant PoIFN-beta fully protected PK15 cells against 1000 TCID50 of porcine transmissible gastroenteritis virus and pseudo-rabies virus infection, demonstrating its high potential in therapeutic applications. This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-beta. (C) 2010 Published by Elsevier Ltd.
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