Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-beta

文献类型: 外文期刊

第一作者: Chen, Weiye

作者: Chen, Weiye;Hu, Qianqian;Qu, Linmao;Li, HaoBo;Huang, Kehe;Chen, Weiye;Cao, Wenyan;Zhao, Huijun;Hu, Qianqian;Qu, Linmao;Hu, Sen;Ge, Jinying;Wen, Zhiyuan;Wang, Xijun;Li, HaoBo;Bu, Zhigao

作者机构:

关键词: Porcine interferon-beta;CHO-K1 cells;Stable expression;Biological activity;Antiviral activity

期刊名称:CYTOKINE ( 影响因子:3.861; 五年影响因子:4.257 )

ISSN: 1043-4666

年卷期: 2011 年 54 卷 3 期

页码:

收录情况: SCI

摘要: A CHO cell clone (CHO-PoIFN-beta) with stable porcine IFN-beta expression under control of CMV promoter was selected under G418 pressure. In a 25 cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-beta reached 2.3 x 10(6) IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-beta could induce the expression of porcine Mx protein in PK15 cells, and activate the chicken Mx promoter-controlled luciferase reporter gene expression, confirming that the recombinant PoIFN-beta has the biological activity of natural porcine type-I interferon. In addition, the recombinant PoIFN-beta fully protected PK15 cells against 1000 TCID50 of porcine transmissible gastroenteritis virus and pseudo-rabies virus infection, demonstrating its high potential in therapeutic applications. This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-beta. (C) 2010 Published by Elsevier Ltd.

分类号:

  • 相关文献

[1]Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design. Xu, Yan-Zhao,Zhou, Yan-Jun,Zhang, Shan-Rui,Jiang, Yi-Feng,Tong, Wu,Yu, Hai,Tong, Guang-Zhi.

[2]Modification of vectors for functional genomic analysis in plants. Li, J. T.,Yu, G.,Sun, X. H.,Jia, C. G.,Pan, H. Y.,Du, Q.,Li, Q. Y.. 2014

[3]EVALUATION OF ENDOGENOUS REFERENCE GENES OF BACTROCERA (TETRADACUS) MINAX BY GENE EXPRESSION PROFILING UNDER VA RIOUS EXPERIMENTAL CONDITIONS. Wang, Liu-Hao,Dai, Rui-Lin,Zhang, Gui-Fen,Guo, Jian-Ying,Wan, Fang-Hao,Guo, Jian-Ying,Wan, Fang-Hao,Wang, Liu-Hao.

[4]Design, Synthesis, and Antiviral, Fungicidal, and Insecticidal Activities of Tetrahydro-beta-carboline-3-carbohydrazide Derivatives. Liu, Yongxian,Song, Hongjian,Huang, Yuanqiong,Liu, Yuxiu,Li, Yongqiang,Wang, Qingmin,Li, Jiarui,Yang, Peiwen,Shang, Hui,Song, Yuchuan,Xiao, Zhixin. 2014

[5]Specific ligands for classical swine fever virus screened from landscape phage display library. Yin, Long,Luo, Yuzi,Du, Min,Qiu, Hua-Ji,Yin, Long,Liang, Bo,Wang, Fei,Liu, Aihua,Yin, Long,Wang, Fei,Liu, Aihua,Petrenko, Valery A..

[6]Identification, expression profiling of a grass carp TLR8 and its inhibition leading to the resistance to reovirus in CIK cells. Chen, Xiaohui,Yang, Chunrong,Rao, Youliang,Li, Qingmei,Wan, Quanyuan,Peng, Limin,Su, Jianguo,Wang, Qing,Wu, Shuqin.

[7]Expression analyses and antiviral properties of the Beijing-You and White Leghorn myxovirus resistance gene with different amino acids at position 631. Du, L. X.,Zhao, G. P.,Zhang, C. S..

[8]Antiviral Activity of The Host Restriction Factor Tetherin. Yin Xin,Wang Xiao-Jun,Yin Xin,Wei Ping. 2014

[9]Antiviral potency and functional analysis of tetherin orthologues encoded by horse and donkey. Yin, Xin,Gu, Qinyong,Wei, Ping,Yin, Xin,Guo, Miaomiao,Gu, Qinyong,Wu, Xingliang,Wang, Xiaojun. 2014

[10]Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. Jing, Jiao,Shi, Baojun,Xu, Qianqian,Zhou, Bin,Chen, Puyan,Li, Wenliang,Liu, Ke,Ma, Zhiyong. 2015

[11]Antiviral activity of Eupatorium adenophorum leaf extract against tobacco mosaic virus. Hou, Lingyu,Zhang, Miaozhi,Xie, Xiangming,Tian, Zhaofeng,Cao, Aocheng. 2014

[12]Cloning, expression and antiviral activity of mink alpha-interferons. Zhang, Hai-ling,Zhao, Jian-jun,Chai, Xiu-li,Zhang, Lei,Bai, Xue,Hu, Bo,Liu, Hao,Yan, Xi-jun,Zhang, Dong-liang,Ye, Ming,Wu, Wei. 2015

[13]Antiviral activity of doxycycline against vesicular stomatitis virus in vitro. Wu, Zhuan-chang,Wei, Jian-chao,Li, Bei-bei,Shao, Dong-hua,Li, Yu-ming,Liu, Ke,Shi, Yuan-yuan,Qiu, Ya-feng,Ma, Zhi-yong,Wang, Xin,Zhou, Bin. 2015

[14]Lonicera japonica Thunb.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. Shang, Xiaofei,Li, Maoxing,Li, Maoxing,Ding, Hong,Shang, Xiaofei,Pan, Hu,Miao, Xiaolou. 2011

[15]Swine interferon-induced transmembrane protein, sIFITM3, inhibits foot-and-mouth disease virus infection in vitro and in vivo. Xu, Jinfang,Qian, Ping,Wu, Qunfeng,Liu, Shasha,Fan, Wenchun,Wang, Rong,Zhang, Huawei,Chen, Huanchun,Li, Xiangmin,Xu, Jinfang,Qian, Ping,Wu, Qunfeng,Liu, Shasha,Fan, Wenchun,Wang, Rong,Zhang, Huawei,Chen, Huanchun,Li, Xiangmin,Zhang, Keshan. 2014

[16]A conjugate protein containing HIV TAT, ISG20, and a PRRSV polymerase binding inhibits PRRSV replication and may be a novel therapeutic platform. Liu, Ke,Li, Yuming,Huan, Beili,Shao, Donghua,Wei, Jianchao,Qiu, Yafeng,Li, Beibei,Tong, Guangzhi,Ma, Zhiyong,Zhou, Bin,Wang, Feifei,Qian, Yingjuan,Jung, Yong-Sam,Miao, Denian.

[17]Cloning and expression of mink (Neovison vison) interferon-gamma gene and development of an antiviral assay. Zhang, Hailing,Zhao, Jianjun,Bai, Xue,Zhang, Lei,Hu, Bo,Liu, Hao,Xu, Shujuan,Yan, Xijun,Zhang, Hailing,Yan, Xijun,Zhang, Dongliang,Fan, Sining.

[18]Extracellular expression and antiviral activity of a bovine interferon-alpha through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Gang,Wang, Yanqun,Chen, Weiye,Zhang, Lu,Liu, Yonggang,Jiang, Chenggang,Wang, Shujie,Bu, Zhigao,Cai, Xuehui.

[19]IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis. Fu, Jian Ping,Chen, Shan Nan,Zou, Peng Fei,Guo, Zheng,Nie, Pin,Fu, Jian Ping,Huang, Bei,Nie, Pin,Zeng, Ling Bing,Qin, Qi Wei. 2014

[20]Short communication: antiviral activity of porcine IFN-lambda 3 against porcine epidemic diarrhea virus in vitro. Shen, Haiyan,Zhang, Chunhong,Liu, Zhicheng,Sun, Minhua,Sun, Junying,Li, Linlin,Dong, Jiawen,Zhang, Jianfeng,Guo, Pengju.

作者其他论文 更多>>