Development of a Paper-Based Microfluidic Chip for Point-of-Care Detection of PEDV

文献类型： 外文期刊

第一作者： Li, Renfeng

作者： Li, Renfeng;Cao, Wenyan;Jiang, Jiaxin;Yuan, Jiakang;Li, Linyue;Wang, Ziliang;Tian, Xiangqin;You, Yonghe;Zhou, Yanlin;Wang, Fangyu

作者机构：

关键词： porcine epidemic diarrhea virus; loop-mediated isothermal amplification; paper-based microfluidic analytical device; point-of-care detection

期刊名称：VETERINARY SCIENCES （ 影响因子：2.3； 五年影响因子：2.4 ）

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年卷期： 2025 年 12 卷 5 期

页码：

收录情况： SCI

摘要： PEDV poses a significant threat to the global swine industry, necessitating rapid and accurate diagnostic methods for effective disease management. In this study, we developed a foldable, easy-to-use paper-based microfluidic analytical device (mu PAD) for on-site detection of PEDV. The device seamlessly integrates paper-based nucleic acid enrichment, LAMP reaction, and visual lateral flow detection into a single platform. Key parameters, including nucleic acid extraction protocols, chromatographic channel configurations, colorimetric indicators, and reaction temperature and duration, were systematically optimized. The resulting LAMP-mu PAD assay detects PEDV within 30 min at 60 degrees C, achieving a limit of detection of 4.82 x 102 copies/mu L with no cross-reactivity against other viruses. When evaluated against RT-PCR using clinical specimens, the assay demonstrated a specificity of 100%, a sensitivity of 95.3%, and an overall concordance of 98.5%. This paper-based sensor offers a promising alternative for the rapid, on-site detection of PEDV and other highly transmissible pathogens.

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