Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence
文献类型: 外文期刊
第一作者: Yu Xian-chang
作者: Yu Xian-chang;Lin Ling-ling;Shi Qing-hua;Qin Ai-guo;Wang Hua-sen
作者机构:
关键词: potato;GDP-mannose pyrophosphorylase;ascorbate;senescence
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2011 年 10 卷 4 期
页码:
收录情况: SCI
摘要: GDP-D-mannose pyrophosphorylase (GMPase) catalyses the synthesis of GDP-D-mannose and represents the first committed step in the synthesis of ascorbate. In the present study, the GMPase gene of tomato was introduced into potato by Agrobacterium-mediated transformation. Two transgenic lines with higher GMPase expression were selected using qPCR and protein blot analyses. The results showed that the content of L-ascorbic acid (AsA) and the ratio of AsA/DHA (dehydroascorbate) significantly increased in both leaves and tubers of transgenic potato plants. Both pigment content and photosynthetic rate were much higher in transgenic plants than in wild-type plants. Transgenic plants showed a distinguishable change in phenotype from the wild-type plants. Furthermore, transgenic plants showed delayed senescence.
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