Inhibitory effects of Vitamin E on UVB-induced apoptosis of chicken embryonic fibroblasts

文献类型： 外文期刊

第一作者： Jin, Dapeng P.

作者： Jin, Dapeng P.;Cong, Yimei;Zhang, Wenxiu X.;Guan, Weijun;Ma, Yuehui;Jin, Dapeng P.;Yang, Hongjian;Li, Chunying

作者机构：

关键词： apoptotic model;Beijing fatty chicken;Ca2+;embryonic fibroblast line;ultraviolet light;Vitamin E

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

ISSN： 1065-6995

年卷期： 2011 年 35 卷 4 期

页码：

收录情况： SCI

摘要： Apoptosis research has been focused on several model species in the past decades, whereas studies concerned with non-mammalian vertebrate, particularly birds, have rarely been involved. In accord with requirements to expand the biodiversity of apoptotic research, a chicken embryonic fibroblasts model involving UVB (ultraviolet B) as the death stimulus was established through primary explantation and serial passage. Myriads of antioxidants can inhibit UVB-induced apoptosis by virtue of scavenging reactive oxygen species. To improve our understanding of the possible antiapoptotic effects and mechanisms of Vitamin E against UVB-induced apoptosis in chicken embryonic fibroblasts, cells treated with Vitamin E after UVB irradiation were stained with AO/EB and Fluo-3/AM to visualize chromatin distribution and calcium homoeostasis, respectively. They were also analysed by flow cytometry to detect mitochondrial transmembrane potential, and cell cycle progression and apoptotic rates were recorded. RT-PCR was used to analyse the expression of some apoptosis-related genes. Typical apoptotic events, including cell shrinkage, blebbing and nuclear condensation, occurred after radiation. In the presence of Vitamin E following irradiation, apoptotic cells were reduced. Ca2+ release was temporarily prevented, and cell cycle arrest at S/G2 checkpoint had almost completely reverted to normal. fas decreased, while procaspase-3 remained nearly, unchanged with and without Vitamin E, and bcl2/bax ratio was up-regulated, indicating possible anti-apoptotic mechanisms through the mitochondrial pathway. This new investigation of an apoptosis model involving chicken embryonic fibroblasts expands the database of knowledge across a wider spectrum of vertebrate species.

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