Identification, characterization, and expression of a novel P450 gene encoding CYP6AE25 from the Asian corn borer, Ostrinia furnacalis

文献类型： 外文期刊

第一作者： Wang, Jian-hua

作者： Wang, Jian-hua;Liu, Zhi-xin;Kulye, Mahesh;Zeng, Hong-mei;Yang, Feng-shan;Zhang, Yi-tong;Xiao, Luo

作者机构：

关键词： cytochrome P450;real-time PCR;bioinformatics

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2011 年 11 卷

页码：

收录情况： SCI

摘要： An allele of the cytochrome P450 gene, CYP6AE14, named CYP6AE25 (GenBank accession no. EU807990) was isolated from the Asian corn borer, Ostrinia furnacalis (Guenee) (Lepidoptera: Pyralidae) by RT-PCR. The cDNA sequence of CYP6AE25 is 2315 bp in length and contains a 1569 nucleotides open reading frame encoding a putative protein with 523 amino acid residues and a predicted molecular weight of 59.95 kDa and a theoretical pI of 8.31. The putative protein contains the classic heme-binding sequence motif F x x G x x x C x G (residues 451-460) conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 52% identity with the previously published sequence of CYP6AE14 (GenBank accession no. DQ986461) from Helicoverpa armigera. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that CYP6AE25 has a closer phylogenetic relationship with CYP6AE14 and CYP6B1 that are related to metabolism of plant allelochemicals, CYP6D1 which is related to pyrethroid resistance and has a more distant relationship to CYP302A1 and CYP307A1 which are related to synthesis of the insect molting hormones. The expression level of the gene in the adults and immature stages of O. furnacalis by quantitative real-time PCR revealed that CYP6AE25 was expressed in all life stages investigated. The mRNA expression level in 3(rd) instar larvae was 12.8- and 2.97-fold higher than those in pupae and adults, respectively. The tissue specific expression level of CYP6AE25 was in the order of midgut, malpighian tube and fatty body from high to low but was absent in ovary and brain. The analysis of the CYP6AE25 gene using bioinformatic software is discussed.

分类号：

  • 相关文献

[1]The cross-resistance patterns and biochemical characteristics of an imidadoprid-resistant strain of the cotton aphid. Chen, Xiaokun,Wang, Jie,Wang, Kaiyun,Xia, Xiaoming,Shi, Xugen,Wang, Hongyan.

[2]Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton. Zhou, Kexue,Long, Lu,Sun, Quan,Wang, Weina,Gao, Wei,Cai, Chaowei,Mo, Jianchuan,Cheng, Jieru,Zhang, Xiangrui,Liu, Yujia,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Du, Xiongming,Shi, Yuzhen,Yuan, Youlu,Chu, Zongyan.

[3]BRITTLE SHEATH1 encoding OsCYP96B4 is involved in secondary cell wall formation in rice. Wang, Xiaole,Cheng, Zhijun,Zhao, Zhichao,Gan, Lu,Qin, Ruizhen,Ma, Weiwei,Wang, Jiulin,Zhai, Huqu,Wan, Jianmin,Zhou, Kunneng,Zhang, Baocai,Zhang, Baocai.

[4]Identification and functional characterization of novel feline cytochrome P450 2A. Okamatsu, Gaku,Komatsu, Tetsuya,Onaga, Takenori,Uchide, Tsuyoshi,Endo, Daiji,Kirisawa, Rikio,Yin, Guojun,Inoue, Hiroki,Kitazawa, Takio,Teraoka, Hiroki,Kubota, Akira,Yin, Guojun,Uno, Yasuhiro.

[5]Loss of Function of the Cytochrome P450 Gene CYP78B5 Causes Giant Embryos in Rice. Chen, Yilin,Liu, Linglong,Shen, Yingyue,Liu, Shijia,Huang, Jiexue,Long, Qizhang,Wu, Wei,Yang, Chunyan,Chen, Hong,Jiang, Ling,Wan, Jianmin,Guo, Xiuping,Cheng, Zhijun,Wan, Jianmin.

[6]Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis. Huang, Xinzheng,Jing, Weixia,An, Xingkui,Zhang, Qiang,Zhang, Hong,Zhang, Yongjun,Guo, Yuyuan,Jing, Weixia,Zhou, Jingjiang. 2018

[7]Acute toxicity, bioconcentration, elimination and antioxidant effects of fluralaner in zebrafish, Danio rerio. Jia, Zhong-Qiang,Liu, Di,Sheng, Cheng-Wang,Chen, Yu-Ming,Han, Zhao-Jun,Zhao, Chun-Qing,Casida, John E.,Wang, Chen,Song, Ping-Ping. 2018

[8]Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China. Yang, Xin,Zhou, Xiaomao,Yang, Xin,Xie, Wen,Wang, Shao-li,Wu, Qing-jun,Pan, Hui-peng,Li, Ru-mei,Yang, Ni-na,Liu, Bai-ming,Xu, Bao-yun,Zhang, You-jun. 2013

[9]Differential analysis of the cytochrome p450 acaricide-resistance genes in Panonychus citri (Trombidiformes: Tetranychidae) strains. Jiang, Gaofei,Chen, Fei,Ran, Chun,Zhang, Yunfei,Li, Junli,Li, Xiaojiao,Yue, Jiansu,Ran, Chun,Jiang, Gaofei,Zhang, Yunfei,Chen, Fei,Li, Junli,Li, Xiaojiao,Yue, Jiansu,Liu, Haoqiang,Li, Hongjun,Ran, Chun.

[10]Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.). Deng, Wei,Li, Xuefeng,Zheng, Mingqi,Yu, Qin,Bai, Lianyang. 2016

[11]Transcriptome-wide analysis reveals candidate genes responsible for the asymmetric pigment pattern in scallop Patinopecten yessoensis. Sun, X. J.,Zhou, L. Q.,Liu, Z. H.,Wu, B.,Yang, A. G.. 2016

[12]Knockdown of several components of cytochrome P450 enzyme systems by RNA interference enhances the susceptibility of Helicoverpa armigera to fenvalerate. Zhao, Chunqing,Feng, Xiaoyun,Qiu, Lihong,Tang, Tao,Liu, Xueyuan. 2012

[13]CYP287A1 is a carotenoid 2-beta-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1. Zhou, Zhengfu,Zhang, Wei,Su, Shiyou,Chen, Ming,Lu, Wei,Lin, Min,Xu, Yuquan,Molnar, Istvan.

[14]Isolation and Expression Analysis of CYP9A11 and Cytochrome P450 Reductase Gene in the Beet Armyworm (Lepidoptera: Noctuidae). Feng, Xiaoyun,Tang, Tao,Qiu, Lihong,Zhao, Chunqing,Tang, Tao. 2015

[15]Control of Rice Embryo Development, Shoot Apical Meristem Maintenance, and Grain Yield by a Novel Cytochrome P450. Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Liu, Jiyun,Wang, Junmin,Zhang, Xiaoming,Xu, Yonghan,Zhang, Shubiao.

[16]Transcriptomic responses to different doses of cycloxaprid involved in detoxification and stress response in the whitebacked planthopper, Sogatella furcifera. Yang, Yuanxue,Zhang, Yixi,Yang, Baojun,Liu, Zewen,Fang, Jichao.

[17]Protective action of the phyllanthin against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio. Jia, Rui,Liu, Ying-Juan,Xu, Pao,Yin, Guo-Jun,Jia, Rui,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun.

[18]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[19]Exploring laccase genes from plant pathogen genomes: a bioinformatic approach. Peng, B. Z.,Li, P. Q.,Fu, L.,Yu, X. M.. 2015

[20]Sequence analysis of a putative goose RIG-I gene. Li, Guoqin,Li, Jinjun,Tian, Yong,Wang, Deqian,Shen, Junda,Tao, Zhengrong,Xu, Jian,Lu, Lizhi. 2012

作者其他论文 更多>>

微信小程序