Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer

文献类型： 外文期刊

第一作者： Ju Hui-ming

作者： Ju Hui-ming;Bai Li-jing;Ren Hong-yan;Mu Yu-lian;Yang Shu-lin;Li Kui;Ju Hui-ming

作者机构：

关键词： type-A spermatogonia;transgenic mice;enhanced green fluorescent protein;type-A spermatogonia-mediated gene transfer

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals. In our current study, we explored the feasibility of producing stable transgenic mice using these cells. Enhanced pEGFP-N1 plasmids were suspended in ExGen500 transfection reagent and injected at different angles into the testes of 7-d-old male ICR mice. The resulting type-A spermatogonia-mediated gene transfer (TASMGT) mice were then mated with normal females at different stages of sexual maturity (6, 12, and 24 wk). The integration and expression of the introduced EGFP gene was evaluated in the F(1) transgenic offspring by PCR and Southern blotting analysis. The foreign gene integration rates for a low-dose group (15 mu L gene suspension injected into each testis) and a high-dose group (30 mu L suspensions injected) at the three stages of female sexual maturity tested were 11.76% (2/17), 14.29% (3/21), and 11.11% (2/18), and 5% (1/20), 5.56% (1/18), and 0 (0/17), respectively. The average integration rates for these two dose groups were 12.5% (7/56) and 3.64% (2/55), respectively, which was a significant difference (P<0.05). Semi-quantitative RT-PCR analysis further showed that the introduced GFP gene was expressed in 3/9 integration mice. In addition, GFP expression was observed in the sperm cells from the TASMGT mice, and also in the embryos and F(2) pups from the F(1) generation transgenic mice. Hence, although the foreign gene integration rate for TASMGT is not high and the transgenic offspring show as yet unexplained defects, our results indicate that this method is a potentially feasible and reproducible new approach to creating transgenic mice.

分类号：

  • 相关文献

[1]Generation of chimeric minipigs by aggregating 4-to 8-cell-stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein. Ji, Huili,Long, Chuan,Feng, Chong,Shi, Ningning,Jiang, Yingdi,Zeng, Guomin,Li, Xirui,Wu, Jingjing,Pan, Dengke,Ji, Huili,Lu, Shengsheng,Long, Chuan,Lu, Lin. 2017

[2]A simplified system for generating recombinant E3-deleted canine adenovirus-2. Yu, Zuo,Jiang, Qian,Liu, Jiasen,Guo, Dongchun,Quan, Chuansong,Li, Botao,Qu, Liandong. 2015

[3]Silencing effect of shRNA expression vectors with stem length of 21, 27 or 29 bp. Xiao, Hongwei,Zheng, Xinmin,Qiao, Xianfeng,Liu, Zhonghua,Wang, Huayan,Xiao, Hongwei,Zheng, Xinmin,Qiao, Xianfeng. 2011

[4]Using Hygromycin Phosphotransferase and Enhanced Green Fluorescent Protein Genes for Tracking Plastid Transformation in Rice (Oryza sativa L.) via Gold Particle Bombardment. Tang, Ning,Hu, Yuanyi,Xia, Yumei,Cao, Mengliang,Li, Ding,Li, Ding,Tang, Ning,Cao, Mengliang,Liu, Moxuan,Shen, Chunxiu,Cao, Mengliang. 2016

[5]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[6]The aflatoxin-detoxifizyme specific expression in mouse parotid gland. Guan, Li-zeng,Cai, Jin-shun,Yu, Long-zheng,Sun, Yu-ping,Wu, Han-dong,Zhang, Yong-liang,Xi, Qian-yun.

[7]Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina. Meng, Qingfeng,Xiao, Zheng,Yuan, Huiping,Zhou, Xinrong,Yang, Binbin,Sun, Jingbo,Meng, Bo,Sun, Xian,Cheng, Fang,Xue, Fei,Zhu, Yuanmao.

[8]Intestine-specific expression of the beta-glucanase in mice. Guan, Li-Zeng,Xi, Qian-Yun,Wang, Jing-Lan,Zhou, Jun-Yun,Shu, Gang,Jiang, Qing-Yan,Zhang, Yong-Liang,Sun, Yu-Ping,Guan, Li-Zeng. 2014

[9]Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice. Ma, Dezun,Gao, Pengfei,Wang, Qingqing,Jiang, Shengwang,Xiao, Gaojun,Cui, Wentao,Qian, Lili,Cai, Chunbo. 2015

[10]Production of transgenic mice carrying the Thanatin gene by intratesticular injection. Miao, Xiang-Yang,Zhang, Xin. 2011

[11]Study of The Transgenic Efficiency in Different Spermiogenesis Stages in Mice. Zhang Xin,Miao Xiang-Yang,Qu Zhao-Jie,Zhang Qiu-Ting,Zhang Xin,Yin Xun-He,Ma Yan-Fang,Qu Zhao-Jie. 2009

[12]Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site. Qian Li-li,Cai Chun-bo,An Xiao-rong,Ma De-zun,Gao Peng-fei,Jiang Sheng-wang,Wang Qing-qing,Xiao Gao-jun,Cui Wen-tao. 2016

[13]beta-Glucanase specific expression in the parotid gland of transgenic mice. Guan, Li-zheng,Zhang, Yong-liang,Guan, Li-zheng,Sun, Yu-ping,Xi, Qian-yun,Wang, Jing-lan,Zhou, Jun-yun,Shu, Gang,Jiang, Qing-yan,Zhang, Yong-liang,Zhang, Yong-liang. 2013

[14]The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo. Bai Li-jing,Ju Hui-ming,Mu Yu-Lian,Yang Shu-lin,Ren Hong-yan,Ao Hong,Li Kui,Bai Li-jing,Wang Chu-duan,Ju Hui-ming. 2014

[15]Functional verification of a porcine myostatin propeptide mutant. Ma, Dezun,Jiang, Shengwang,Gao, Pengfei,Wang, Qingqing,Xiao, Gaojun,Cui, Wentao,Qian, Lili,Cai, Chunbo,Yang, Jinzeng.

作者其他论文 更多>>

微信小程序