Identification of a linear epitope on the capsid protein of classical swine fever virus

文献类型: 外文期刊

第一作者: Zhang, Xin

作者: Zhang, Xin;Sun, Yuan;Li, Su;Li, Na;Yang, Shuo;He, Fan;Huang, Jun-Hua;Ling, Li-Jun;Qiu, Hua-Ji;Xu, Jia

作者机构:

关键词: Classical swine fever virus;Capsid protein;Monoclonal antibody;Epitope;Subcellular localization

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN: 0168-1702

年卷期: 2011 年 156 卷 1-2 期

页码:

收录情况: SCI

摘要: The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication. (C) 2011 Elsevier B.V. All rights reserved.

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