Evaluation of recombinant fowlpox virus expressing infectious bronchitis virus S1 gene and chicken interferon-gamma gene for immune protection against heterologous strains

文献类型： 外文期刊

第一作者： Shi, Xing-Ming

作者： Shi, Xing-Ming;Zhao, Yan;Gao, Hong-Bo;Jing, Zhang;Wang, Mei;Cui, Hong-Yu;Tong, Gang-Zhi;Wang, Yun-Feng

作者机构：

关键词： Infectious bronchitis virus;Recombinant fowl poxvirus;IFN-gamma;S1 protein

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2011 年 29 卷 8 期

页码：

收录情况： SCI

摘要： A recombinant fowlpox virus (rFPV-IFN gamma S1) that co-expressed the infectious bronchitis virus (IBV) S1 gene and the chicken interferon-gamma gene has been constructed. To evaluate the efficacy of the recombinant fowlpox virus vaccine against heterotypic IBV strains, 60 4-week-old Specific-Pathogen-Free (SPF) chickens were inoculated with this vaccine and 3 weeks post inoculation challenged with the homotypic IBV strain LX4 and the heterotypic IBV strains LHB, LHLJO4XI, LTJ95I and LSC99I. Antibodies against IBV were detected in vaccinated chickens 1-week post inoculation. The number of CD4(+). and CD8(+) T-lymphocytes in the peripheral blood increased rapidly in the vaccinated groups challenged with strains LX4, LHB and LHLJO4XI. There were significant differences in the number of CD4(+) and CD8(+) T-lymphocytes between the vaccinated groups challenged with strains LTJ95I and LSC99I and all the control groups. The morbidity was below 30% in vaccinated groups challenge with strains LX4, LHB and LHLJO4XI, but was 40% greater than that in the other groups. In addition, the lesions and the amount of virus shedding were less severe in the vaccinated groups challenged by strains LX4, LHB and LHLJO4X1 when compared with the other groups, but there was no significant difference in the average body weight of the chickens in all groups (all p > 0.05). These results indicate that the rFPV-IFN gamma S1 protected chickens against challenge with homotypic IBV strain LX4 and heterotypic strains LHLJO4XI and LHB. (C) 2011 Elsevier Ltd. All rights reserved.

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