Cloning and functional analysis of a new laccase gene from Trametes sp 48424 which had the high yield of laccase and strong ability for decolorizing different dyes
文献类型: 外文期刊
第一作者: Fan, Fangfang
作者: Fan, Fangfang;Zhuo, Rui;Sun, Su;Zhang, Xiaoyu;Yang, Yang;Fan, Fangfang;Zhuo, Rui;Wan, Xia;Jiang, Mulan;Yang, Yang
作者机构:
关键词: White-rot fungi;Laccase gene;Heterologous expression;cis-Acting elements;Decolorization of dyes
期刊名称:BIORESOURCE TECHNOLOGY ( 影响因子:9.642; 五年影响因子:9.237 )
ISSN: 0960-8524
年卷期: 2011 年 102 卷 3 期
页码:
收录情况: SCI
摘要: The laccase gene lac48424-1 and its corresponding full-length cDNA were cloned and characterized from a novel white-rot fungi Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. The 1563 bp full-length cDNA of lac48424-1 encoded a mature laccase protein containing 499 amino acids preceded by a signal peptide of 21 amino acids. The deduced protein sequence of LAC48424-1 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. The functionality of lac48424-1 gene encoding active laccase was verified by expressing the gene in the yeast Pichia pastoris successfully. It was found that the recombinant laccase produced by the yeast transformant could decolorize different dyes. The 5'-flanking sequence upstream of start codon was obtained by Self-Formed Adaptor PCR. Many putative cis-acting responsive elements involved in the transcriptional regulation were identified in the promoter region of lac48424-1. (C) 2010 Elsevier Ltd. All rights reserved.
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