Studies on the DNA Barcoding of Two Newly Discovered Chicken Breeds by mtDNA COI Gene

文献类型： 外文期刊

第一作者： Gao Yu-Shi

作者： Gao Yu-Shi;Tu Yun-Jie;Tang Xiu-Jun;Lu Jun-Xian;Zhang Xiao-Yan

作者机构：

关键词： Indigenous chicken breeds;mtDNA COI gene;DNA barcoding;variety identification;genetics;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2011 年 10 卷 13 期

页码：

收录情况： SCI

摘要： In order to investigate the genetic diversity of two newly discovered chicken breeds (Jinhu silky and Wahui) and their genetic relationships with other indigenous chicken breed (Langshan), the COI gene of these three breeds were analyzed by amplification and direct sequencing. The results showed that the selectived sequence of COI had ten SNPs and seven haplotypes, three of SNPs were specific mutation sites, five of haplotypes were specific haplotypes. The Pi of Jinhu silky, Wahui and Langshan were 0.068, 0.051 and 0.369%, respectively and the Hd were 0.4167, 0.3030 and 0.9000, respectively. The Kimura 2-parameter distances between breeds ranged from -0.002 to 0.236. The genetic distances of intrapopulation were less than that of interpopulation. The data suggest that the genetic diversity of Langshan was higher than that of two newly discovered breeds, the specific mutation sites and haplotypes provide the gist for variety identification.

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