Research on Determination of Lecithin in Eggs by Applying Microwave Digestion Techniques and Spectrophotometry

文献类型： 外文期刊

第一作者： Wu Min

作者： Wu Min;Ge Qing-lian;Gao Yu-shi;Chen Kuan-wei

作者机构：

关键词： Spectrophotometry;Microwave digestion;Phosphatidylcholine

期刊名称：IRANIAN JOURNAL OF CHEMISTRY & CHEMICAL ENGINEERING-INTERNATIONAL ENGLISH EDITION （ 影响因子：0.759； 五年影响因子：1.018 ）

ISSN： 1021-9986

年卷期： 2012 年 31 卷 2 期

页码：

收录情况： SCI

摘要： A method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. The homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. A sample then received a new way of pre-treatment, called microwave digestion, before UV-Vis spectrometry was applied to examine the concentration of phosphate at 400nm. The linear equation was A = 0.08628X (mu g), the corresponding coefficient of correlation was 0.9998, the detection limit of phosphorous was 0.2 mu g (n=11). The content of lecithin in eggs was then obtained. According to the result, the recovery of 90% was secured; therefore the conclusion of high degree of accuracy was reached.

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