The Apoptotic Effects of Alcohol Shock on Bovine Oocytes

文献类型: 外文期刊

第一作者: Li, Xiangchen

作者: Li, Xiangchen;Yao, Yaxin;Liu, Peng;Jin, Dapeng;Guan, Weijun;Yuehui, M. A.;Yao, Yaxin;Jin, Dapeng

作者机构:

关键词: Alcohol shock;apoptosis;bovine;oocyte;ovaries;China

期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )

ISSN: 1680-5593

年卷期: 2011 年 10 卷 20 期

页码:

收录情况: SCI

摘要: Bovine oocytes collected from slaughterhouse should be subject to rigorous antiseptic procedures. In this lab, alcohol immersion was applied for preventing microbial infection instead of antibiotics. However, it remains to be investigated whether alcohol treatment would lead to unpredictable results. In the present study, the ovaries were fully washed with 75% alcohol for 1, 5 and 10 min, respectively. Only in the 10 min group, the oocytes apoptotic rates significantly increased (p<0.05) and the development rates significantly decreased (p<0.01) while detectable difference in apoptosis was not observed. Therefore, washing ovaries with 75% alcohol for <5 min constitutes no obvious influences on the development of oocytes and embryos and could be used as a convenient and rapid method to prevent microbial contamination.

分类号:

  • 相关文献

[1]Effect of mouse cumulus cells on the in vitro maturation and developmental potential of bovine denuded germinal vesicle oocytes. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Liu, Yan,Qin, Tong,Wang, Dong,Zhu, Hua-Bin.

[2]Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro. Li Xiangchen,Yao Yaxin,Wang Yipeng,Li Heping,Guan Weijun,Ma Yuehui. 2012

[3]The relationships between gonad development and sex steroid levels at different ages in Acipenser schrenckii. Qu, Q. -Z.,Sun, D. -J.,Wan, B. -Q.,Ma, G. -J..

[4]Effect of SIRT1 on Apoptosis of Bovine Preadipocytes. Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Wu Gong-Ying. 2010

[5]Fas-Associated Death Domain (FADD) Mediated Activation of a Apoptosis Program in Bovine Follicular Granulosa Cells. Yang, Run-Jun,Li, Jun-Ya,Gao, Xue,Zhang, Lu-Pei,Gao, Hui-Jiang,Xu, Shang-Zhong,Yang, Run-Jun,Zhao, Zhi-Hui. 2011

[6]Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii). Zhang, Y.,Dong, X. L.,Sun, D. J.,Jin, S. B.,Xi, Q. K.,Song, D.,Fu, H. T.. 2015

[7]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[8]Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation. Tan, Xiuwen,Song, Enliang,Liu, Xiaomu,Liu, Guifen,Cheng, Haijian,Wan, Fachun.

[9]Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms. Zhao, Shan-Jiang,Pang, Yun-Wei,Zhao, Xue-Ming,Du, Wei-Hua,Hao, Hai-Sheng,Zhu, Hua-Bin. 2017

[10]Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion. Hongtuo, F,Chingjiang, W. 2001

[11]Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes. Huang, Jing,Li, Qingwang,Li, Wenye,Xiao, Bo,Wu, Shuyun,Jiang, Zhongliang,Hu, Jianhong,Li, Qingwang,Zhao, Rui,Han, Zengsheng,Liu, Lei,Chen, Xiaoyu,Xiao, Bo. 2008

[12]Linear Loading Cryoprotectants (CPAs) With Microfluidic Method Reduces Osmotic Damage to Porcine M II-stage Oocytes. Yang Yun,Zhou Xin-Li,Shag Wen-Qi,Yi Xing-Yue,Tao Le-Ren,Dai Jian-Jun,Zhang De-Fu. 2016

[13]Expression of bone morphogenetic proteins and receptors in porcine cumulus-oocyte complexes during in vitro maturation. Zhu, Guiyu,Pan, Dengke,Mu, Yulian,Feng, Shutang,Zhu, Guiyu,Guo, Bingran. 2008

[14]Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions. Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping,Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping. 2012

[15]Effect of beta-mercaptoethanol and buffalo follicular fluid on fertilization and subsequent embryonic development of water buffalo (Bubalus bubalis) oocytes derived from in vitro maturation. Shang, J. H.,Huang, Y. J.,Zhang, X. F.,Huang, F. X.,Qin, J.. 2007

[16]The Evaluation of Factors Affected on Juvenile in vitro Embryo Transfer in Xinjiang Fine Wool Lamb. Wu, Weiwei,Wang, Feng,Wu, Weiwei,Hanikezi,Tian, Kechuan,Xu, Xinming,Tian, Yuezhen. 2012

[17]The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep. Zhang, Rui-Na,Pang, Bo,Guo, Song-Chang,Jia, Gong-Xue,Hu, Lin-Yong,Zhao, Xin-Quan,Yang, Qi-En,Xu, Shang-Rong,Wan, Peng-Cheng,Ji, Han-Zhong,Yang, Qi-En. 2018

[18]Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes. Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Li, Jingchun.

[19]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[20]Mannose-binding lectin 1 haplotypes influence serum MBL-A concentration, complement activity, and milk production traits in Chinese Holstein cattle. Liu, Jianbo,Ju, Zhihua,Li, Qiuling,Huang, Jinming,Li, Rongling,Li, Jiangbin,Zhong, Jifeng,Wang, Changfa,Liu, Jianbo,Ma, Lijuan. 2011

作者其他论文 更多>>