Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1
文献类型: 外文期刊
第一作者: Jiang, Tao
作者: Jiang, Tao;Liang, Zhong;Ren, Weiwei;Chen, Juan;Zhi, Xiaoying;Qi, Guangyu;Yang, Yamin;Liu, Zaixing;Liu, Xiangtao;Cai, Xuepeng;Ren, Weiwei;Zhi, Xiaoying;Qi, Guangyu
作者机构:
关键词: Colloidal gold particles;Foot-and-mouth disease virus;Lateral flow immunoassay;Serotyping
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN: 0166-0934
年卷期: 2011 年 171 卷 1 期
页码:
收录情况: SCI
摘要: A lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes O. A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples (n = 396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples (n = 95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%. (C) 2010 Elsevier B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Development of an automatic tubular chemiluminescence immunoassay using magnetic particles for the detection of antibodies against foot-and-mouth disease virus serotype A
作者:Li, Fengsong;Cui, Yan;Li, Fengsong;Bao, Yanfang;Jiang, Tao;Li, Taoshan;Zuo, Yili;Yang, Guang;Li, Xin;Lin, Mi;Jiang, Tao;Lin, Mi
关键词:Tubular chemiluminescence immunoassay; Automation; Foot-and-mouth disease virus serotype A; Antibody detection; Serum
-
Phylogeography Analysis Reveals Rabies Epidemiology, Evolution, and Transmission in the Philippines
作者:Zhang, Liang;Jiang, Jianfeng;Gong, Wenjie;Zhang, Liang;Sun, Sheng;Liu, Yan;Tu, Changchun;Gong, Wenjie;Feng, Ye;Cruz, Jeffrey;Gonzales, Riva Marie;Azul, Rachel R.;Pena, Rainelda C. Dela;Tian, Yao;Jiang, Tao;Fang, Liqun;Feng, Ye;Wang, Yuyang;Tu, Changchun
关键词:rabies virus; Philippines; molecular epidemiology; transmission patterns; selection pressure; strategy
-
C-type natriuretic peptide regulates lipid metabolism through a NPRB-PPAR pathway in the intramuscular and subcutaneous adipocytes in chickens
作者:Huang, Huayun;Liu, Longzhou;Liang, Zhong;Wang, Qianbao;Li, Chunmiao;Huang, Zhengyang;Zhao, Zhenhua;Han, Wei;Huang, Huayun;Liu, Longzhou;Liang, Zhong;Wang, Qianbao;Li, Chunmiao;Huang, Zhengyang;Zhao, Zhenhua;Han, Wei
关键词:C-type natriuretic peptide; Intramuscular adipocytes; Subcutaneous adipocytes; PPAR pathway
-
Chromosome-level genome assembly of the freshwater bivalve Anodonta woodiana
作者:Chen, Xiubao;Jiang, Tao;Xue, Junren;Liu, Kai;Gu, Mengying;Wang, Meiyi
关键词:
-
MYB regulation of GST/GT mediates red petal spot development in cotton
作者:Zhang, Sujun;Wang, Haitao;Cai, Xiao;Liu, Cunjing;Tang, Liyuan;Li, Xinghe;Zhang, Jianhong;Chen, Jie;Jiang, Tao;Sun, Yaqian
关键词:Cotton; Red-spot-petal; MYB-encoding gene; Glutathione S transferase gene; Glycosyl-transferase gene
-
Transcriptome sequencing revealed the regulation of stem internode length associated with mechanical harvesting in three tea varieties
作者:Mi, Xiaozeng;Qiao, Dahe;Yang, Chun;Chen, Juan;Liang, Sihui;Guo, Yan;Mi, Xiaozeng;Yang, Chun;Liang, Sihui;Guo, Yan;Qiao, Dahe
关键词:stem internode length; mechanical harvesting; tea plant; RNA-Seq; WGCNA
-
Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of Penaeus semisulcatus
作者:Li, Yundong;Chen, Juan;Jiang, Song;Yang, Qibin;Yang, Lishi;Huang, Jianhua;Shi, Jianzhi;Ding, Yangyang;Zhou, Falin;Li, Yundong;Yang, Qibin;Zhou, Falin;Li, Yundong;Huang, Jianhua;Zhou, Falin;Jiang, Ziyi;Liu, Tianmi
关键词:Penaeus semisulcatus; amino acids; fatty acids; antioxidant enzyme activity; genetic diversity