Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori Densovirus Zhenjiang Strain
文献类型: 外文期刊
第一作者: Zhao Pan
作者: Zhao Pan;Tang Shun-ming;Guo Xi-jie;Tang Shun-ming;Liu Ting;Qin Guang-xing;Guo Xi-jie
作者机构:
关键词: Bombyx mori;densovirus;non-structure protein;expression;activity
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2010 年 9 卷 12 期
页码:
收录情况: SCI
摘要: The gene of the non-structure protein 2 (NS2) was cloned by PCR from the genome of Bombyx mori densovirus Zhenjiang strain (BmDNV-Z), inserted into prokaryotic expression vector pET28a to construct recombinant plasmid pET28a-NS2 and then expressed in bacteria Escherichia coli BL21 (DE3) The expressed recombinant protein was identified by SDS-PAGE and Western blot analysis Then, the recombinant protein was purified by Ni-NTA column, renatured and tested for enzyme activities The purified NS2 protein exhibited a helicase activity unwinding double-stranded DNA substrates into single-strand primers, and higher unwinding activity to polarity substrate Similarly, the purified NS2 protein possessed an ATPase activity and its enzyme activity was 0 276 mu mol mu g (1) h (1) in this study The results indicated that the non-structure protein which encoded by the gene of BmDNV-Z NS2 possesses the biological activities of helicase and ATPase, and the helicase prefers to polarity substrates Based on these results, it is speculated that the gene of BmDNV-Z NS2 plays an important role in the viral DNA replication
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