Properties of a novel alpha-galactosidase from Streptomyces sp. S27 and its potential for soybean processing
文献类型: 外文期刊
第一作者: Cao, Yanan
作者: Cao, Yanan;Yuan, Tiezheng;Shi, Pengjun;Luo, Huiying;Li, Ning;Meng, Kun;Bai, Yingguo;Yang, Peilong;Zhou, Zhigang;Yao, Bin;Zhang, Zhifang
作者机构:
关键词: alpha-Galactosidase;Streptomyces;Overexpression;High hydrolytic activity;Raffinose family oligosaccharides (RFOs)
期刊名称:ENZYME AND MICROBIAL TECHNOLOGY ( 影响因子:3.493; 五年影响因子:3.699 )
ISSN: 0141-0229
年卷期: 2010 年 47 卷 7 期
页码:
收录情况: SCI
摘要: A full-length alpha-galactosidase gene (2226 bp) was cloned from Streptomyces sp. S27 ACCC 41168 and overexpressed in Escherichia coli. The deduced amino acid sequence shared highest identities of 82% with a putative alpha-galactosidase from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111 and 46% with a known alpha-galactosidase from Bifidobacteriurn bifidum NCIMB 41171, both of which belong to glycoside hydrolase (CH) family 36. The purified recombinant enzyme showed a single protein band of similar to 82 kDa on SDS-PAGE and three bands of similar to 220, 320 and 480 kDa on non-denaturing gradient PAGE, respectively, indicating its native structure of trimer, tetramer or hexamer. The enzyme exhibited optimal activity at conditions of 35 degrees C and pH 7.4. similar to the intestinal conditions of mammals and poultry, was resistant to some neutral proteases (alpha-chymotrypsin, subtilisin A and collagenase), and showed hydrolytic ability to natural substrates, including melibiose, stachyose, raffinose and soybean meal. When combined with intestinal proteases, the enzyme showed higher hydrolytic ability to raffinose family oligosaccharides (RFOs) in soybean product. These favorable properties make the Streptomyces sp. S27 alpha-galactosidase very prospective in soybean processing for food and feed industries. (C) 2010 Elsevier Inc. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Effect of crowding stress on liver health, gut permeability and gut microbiota of genetically improved farmed tilapia (GIFT, Oreochromis niloticus)
作者:Zhang, Jian;Chen, Jie;Liang, Hui;Li, Ming;Zhou, Wenhao;Zhou, Zhigang;Yang, Yalin;Zhang, Zhen;Ran, Chao;Ding, Qianwen
关键词:Crowing stress; Apoptosis; Gut permeability; Gut microbiota; GIFT
-
Integrative Analysis of Metabolome and Transcriptome of Carotenoid Biosynthesis Reveals the Mechanism of Fruit Color Change in Tomato (Solanum lycopersicum)
作者:Hu, Jiahui;Yu, Qinghui;Hu, Jiahui;Wang, Juan;Muhammad, Tayeb;Yang, Tao;Li, Ning;Yang, Haitao;Yu, Qinghui;Wang, Baike
关键词:tomato; fruit ripening; metabolome; transcriptome; carotenoids; lycopene; ethenyl
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity
作者:Zhang, Wenrong;Wang, Haining;Wu, Tong;Gao, Xintao;Shang, Yuting;Zhang, Zhifang;Liu, Xingjian;Li, Yinu;Zhang, Wenrong
关键词:COVID-19; ferritin; fenobody; baculovirus expression system; silkworm
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者:He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词:beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
-
Identification of Black Spot Resistance in Broccoli (Brassica oleracea L. var. italica) Germplasm Resources
作者:Zhang, Quan;Li, Ning;Liu, Ning;Ding, Yunhua;Zhang, Quan;Ding, Yunhua;Branca, Ferdinando;Li, Ning;Liu, Ning
关键词:broccoli; black spot disease; germplasm resources; breeding