A new alpha-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for alpha-galactosidase activity of gastrointestinal bacteria
文献类型: 外文期刊
第一作者: Zhou, Junpei
作者: Zhou, Junpei;Shi, Pengjun;Huang, Huoqing;Cao, Yanan;Meng, Kun;Yang, Peilong;Zhang, Rui;Chen, Xiaoyan;Yao, Bin;Zhou, Junpei
作者机构:
关键词: Batocera horsfieldi;Flavobacterium sp.;TN17;alpha-Galactosidase;Symbiotic bacteria;Site-directed mutagenesis
期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )
ISSN: 0175-7598
年卷期: 2010 年 88 卷 6 期
页码:
收录情况: SCI
摘要: The alpha-galactosidase gene, galA17, was cloned from Flavobacterium sp. TN17, a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae. The 2,205-bp full-length gene encodes a 734-residue polypeptide (GalA17) containing a putative 28-residue signal peptide and a catalytic domain belonging to glycosyl hydrolase family 36 (GH 36). The deduced amino acid sequence of galA17 was most similar to a putative alpha-galactosidase from Pedobacter sp. BAL39 (EDM38577; 66.6% identity) and a characterized alpha-galactosidase from Carnobacterium piscicola BA (AAL27305; 30.1%). Phylogenetic analysis revealed that GalA17 was similar to GH 36 alpha-galactosidases from symbiotic bacteria sharing two putative catalytic motifs, KWD and SDXXDXXXR, in which D480, S548, D549, and R556 were essential for alpha-galactosidase activity based on site-directed mutagenesis. Purified recombinant GalA17 showed apparent optimal activity at pH 5.5 and 45A degrees C; exhibited strong resistance to digestion by trypsin, alpha-chymotrypsin, collagenase, and proteinase K; and efficiently hydrolyzed several synthetic and natural substrates (p-nitrophenyl-alpha-d-galactopyranoside, stachyose, melibiose, raffinose, soybean meal, locust bean gum, and guar gum).
分类号:
- 相关文献
作者其他论文 更多>>
-
Ganoderma lucidum Polysaccharides Ameliorate Acetaminophen-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Apoptosis along the Nrf2 Pathway
作者:Zhang, Nan;Liu, Linling;Gao, Yanliang;Li, Jintao;Yan, Meixia;Zhang, Nan;Han, Zhongming;Zhang, Rui;Zhang, Nan;Liu, Linling;Gao, Yanliang;Li, Jintao;Yan, Meixia
关键词:Ganoderma lucidum polysaccharides; acute liver injury; oxidative stress; Nrf2 signaling pathway
-
Influence of Drought Stress on the Rhizosphere Bacterial Community Structure of Cassava (Manihot esculenta Crantz)
作者:Huang, Huling;Li, Mingchao;Guo, Qiying;Zhang, Rui;Luo, Kai;Chen, Yinhua;Huang, Huling;Li, Mingchao;Guo, Qiying;Zhang, Rui;Luo, Kai;Chen, Yinhua;Zhang, Yindong
关键词:drought stress; cassava; rhizosphere bacterial community; environmental factor; drought response
-
Biocontrol performance and mass production potential of the larval endoparasitoid Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
作者:Zhang, Rui;Zhang, Wei;Zhao, Qi;Keyhani, Nemat O.;Lei, Xian-Fu;Liu, Chang-Hua;Al Dhafer, Hathal M.;Mohamed, Amr
关键词:Spodoptera frugiperda; Field investigation; Campoletis chlorideae; Biological control; Mass production; Two-sex life table
-
GLK transcription factors accompany ELONGATED HYPOCOTYL5 to orchestrate light-induced seedling development in Arabidopsis
作者:Zhang, Ting;Zhang, Rui;Zeng, Xi-Yu;Ye, Lu-Huan;Zhu, Xin-Guang;Wang, Peng;Zhang, Ting;Zeng, Xi-Yu;Tian, Shi-Long;Zhang, Rui;Ye, Lu-Huan;Zhu, Xin-Guang;Wang, Peng;Lee, Sanghwa;Busch, Wolfgang;Zhang, Yi-Jing;Zhou, Wen-Bin
关键词:
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Application of Single-Cell Assay for Transposase-Accessible Chromatin with High Throughput Sequencing in Plant Science: Advances, Technical Challenges, and Prospects
作者:Lu, Chao;Wei, Yunxiao;Abbas, Mubashir;Meng, Zhigang;Zhang, Rui;Lu, Chao;Agula, Hasi;Wang, Edwin
关键词:scATAC-seq; plant science; epigenomics; chromatin accessibility; cis-regulatory elements
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词: