Characterization of the biochemical properties and identification of amino acids forming the catalytic center of 3C-like proteinase of porcine reproductive and respiratory syndrome virus
文献类型: 外文期刊
第一作者: Xu, Ao-Tian
作者: Xu, Ao-Tian;Zhou, Yan-Jun;Li, Guo-Xin;Yu, Hai;Yan, Li-Ping;Tong, Guang-Zhi
作者机构:
关键词: 3C-like proteinase;Nsp4;Porcine reproductive and respiratory syndrome virus
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN: 0141-5492
年卷期: 2010 年 32 卷 12 期
页码:
收录情况: SCI
摘要: Purpose of work The non-structural protein 4 (Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV) functions as a 3C-like proteinase (3CLpro) and plays a pivotal role in gene expression and replication. We have examined the biochemical properties of PRRSV 3CLpro and identified those amino acid residues involved in its catalytic activity as a prelude to developing anti-PRRSV strategies. The 3C-like proteinase (3CLpro) of porcine reproductive and respiratory syndrome virus (PRRSV) was expressed in Escherichia coli and characterized. The optimal temperature and pH for its proteolytic activity were 8 degrees C and 7.5, respectively. Na(+) (1000 mM) and K(+) (500 mM) were not inhibitory to its activity but Cu(2+), Zn(2+), PMSF and EDTA were significantly inhibitory. His(39), Asp(64) and Ser(118) residues were identified to form the catalytic triad of PRRSV 3CLpro by a series of site-directed mutagenesis analysis.
分类号:
- 相关文献
作者其他论文 更多>>
-
Japanese encephalitis virus NS3 captures the protein translation element by interacting with HNRNPH1 to promote viral replication
作者:Wang, Xingya;Li, Lin;Liu, Xuelan;Wang, Xingya;Kong, Ning;Wang, Chen;Qin, Wenzhen;Yang, Xinyu;Yu, Hai;Tong, Wu;Tong, Guangzhi;Zheng, Hao;Shan, Tongling;Kong, Ning;Yu, Hai;Tong, Wu;Tong, Guangzhi;Zheng, Hao;Shan, Tongling;Liu, Xuelan
关键词:JEV; NS3; HNRNPH1; Translation regulation element
-
BTG3 inhibits porcine epidemic diarrhea virus replication by promoting viral S2 protein degradation through the autophagy and proteasome pathways
作者:Zhu, Qingxiao;Liu, Xuelan;Kong, Ning;Zhu, Qingxiao;Liu, Tian;Qin, Wenzhen;Yang, Xinyu;Tong, Wu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Shan, Tongling;Kong, Ning;Shan, Tongling;Kong, Ning;Zhang, Yu
关键词:BTG3; PEDV S2 subunit; Degradation; Autophagy pathway
-
RNASEK interacting with PEDV structural proteins facilitates virus entry via clathrin-mediated endocytosis
作者:Qin, Wenzhen;Kong, Ning;Yang, Xinyu;Wang, Yahe;Cao, Xinyu;Liu, Yuchang;Wang, Jiarui;Sun, He;Tong, Wu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Shan, Tongling;Kong, Ning;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Shan, Tongling;Xie, Shengsong;Liu, Hailong;Zhang, Wen
关键词:PEDV; RNASEK; virus entry; endocytosis
-
Midnolin inhibits coronavirus proliferation by degrading viral proteins
作者:Wang, Yahe;Tong, Wu;Qin, Wenzhen;Yang, Xinyu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Wang, Chunmei;Kong, Ning;Shan, Tongling;Tong, Wu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Wang, Chunmei;Kong, Ning;Shan, Tongling;Zhang, Wen
关键词:midnolin; PEDV; proteasome; autophagy; domain
-
POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins
作者:Cao, Xinyu;Liu, Yingyu;Cao, Xinyu;Tong, Wu;Qin, Wenzhen;Yang, Xinyu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Kong, Ning;Shan, Tongling;Zhang, Wen;Kong, Ning;Shan, Tongling
关键词:POLM; PEDV; N protein; S2 protein; M protein; MARCH8; p62; autophagy
-
Development and application of a blocking ELISA method based on Cap protein for detecting antibodies against porcine circovirus 2
作者:Liu, Qingyan;Gao, Shuolei;Yang, Jingyi;Zhu, Yuxiang;Zhou, Yanjun;Shan, Tongling;Tong, Wu;Zheng, Hao;Kong, Ning;Jiang, Yifeng;Liu, Changlong;Tong, Guangzhi;Yu, Hai;Li, Jiannan;Zhu, Jun;Yu, Hai
关键词:porcine circovirus type 2 (PCV2); Cap protein monoclonal antibody; blocking ELISA
-
FSTL1 and TLR4 interact with PEDV structural proteins to promote virus adsorption to host cells
作者:Liu, Chunyun;Kong, Ning;Qin, Wenzhen;Zhao, Wenli;Yang, Xinyu;Wang, Yahe;Cao, Xinyu;Liu, Tian;Liu, Yuchang;Sun, He;Tong, Wu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Shan, Tongling;Liu, Chunyun;Zhao, Wenli;Lan, Daoliang;Kong, Ning;Shan, Tongling;Liu, Hailong;Xie, Shengsong;Liu, Hailong;Xie, Shengsong;Zhang, Yu
关键词:FSTL1; TLR4; PEDV; structural proteins; virus adsorption