Schistosoma japonicum: Cloning, expression and characterization of a gene encoding the alpha 5-subunit of the proteasome

文献类型: 外文期刊

第一作者: Hong, Yang

作者: Hong, Yang;Han, Hongxiao;Peng, Jinbiao;Li, Ye;Shi, Yaojun;Fu, Zhiqiang;Liu, Jinming;Lin, Jiaojiao;Hong, Yang;Han, Hongxiao;Li, Xiangrui

作者机构:

关键词: Schistosoma japonicum (S. japonicum);Proteasome subunit alpha type 5 (PSMA5);Immunity;Vaccine

期刊名称:EXPERIMENTAL PARASITOLOGY ( 影响因子:2.011; 五年影响因子:2.132 )

ISSN: 0014-4894

年卷期: 2010 年 126 卷 4 期

页码:

收录情况: SCI

摘要: The development of an effective vaccine against the schistosome is thought to be the most desirable means to control schistosomiasis, even though there is an effective means of chemotherapy with praziquantel. A full-length cDNA encoding the Schistosoma japonicum proteasome subunit alpha type 5 protein (SjPSMA5) was first isolated from 18-day-schistosomulum cDNAs. The cDNA had an open reading frame (ORF) of 747 bp and encoded 248 amino acids. Real-time quantitative RT-PCR analysis revealed that SjPSMA5 is up-regulated in 18-day and 32-day schistosomes, and the level of expression in male is around fourfold higher than that in female worms at 42 days. The SjPSMA5 was subcloned into pET28a(+) and expressed as inclusion bodies in Escherichia coli BL21 (DE3) cells. Western blotting showed that the recombinant SjPSMA5 (rSjPSMA5) was immunogenic. After immunization of BALB/c mice with rSjPSMA5, reductions of 23.29% and 35.24% were obtained in the numbers of worms and eggs in the liver, respectively. The levels of specific IgG antibodies and CD(4)(+) cells were significantly higher (P < 0.01) in the group vaccinated with rSjPSMA5 combined with Seppic 206 adjuvant than in the other groups, as detected by enzyme linked imrnunosorbent assay (ELISA) and flow cytometry. The study suggested that rSjPSMA5 induced partial immunoprotection against S. japonicurn in BALB/c mice, and it could be a potential vaccine candidate against schistosomiasis. (C) 2010 Elsevier Inc. All rights reserved.

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