Characterization of Streptococcus suis serotype 2 blood infections using RT-qPCR to quantify glutamate dehydrogenase copy numbers
文献类型: 外文期刊
第一作者: Gu, Jiangping
作者: Gu, Jiangping;Li, Caiying;Yue, Xiuwei;Yuan, Congli;Cui, Li;Hua, Xiuguo;Yang, Zhibiao;Cai, Xuehui;Liu, Yonggang;Wang, Shujie;Yang, Weijun;Hao, Yongqing;Xing, Rui;Li, Caiying;Zhang, Mingfeng
作者机构:
关键词: Streptococcus suis serotype 2;TaqMan probe;Real-time quantitative PCR;Blood infections
期刊名称:JOURNAL OF MICROBIOLOGICAL METHODS ( 影响因子:2.363; 五年影响因子:2.24 )
ISSN: 0167-7012
年卷期: 2010 年 83 卷 3 期
页码:
收录情况: SCI
摘要: This study characterized the dynamic distribution of bacteria in the blood of pigs infected with Streptococcus suis serotype 2 using specific primers and a TaqMan probe designed to amplify the highly conserved S. suis serotype 2 glutamate dehydrogenase (GDH) gene sequences. Gene copy numbers were used to determine the concentration of bacteria in the blood of infected pigs over time using established TaqMan real-time quantitative PCR methodologies (RT-qPCR). The results showed that the detection limit of the RT-qPCR was 10 GDH gene copies. The advantages of utilizing this approach are the high levels of specificity, sensitivity and reproducibility. Bacteria were detected in the blood of infected pigs after 24 h post infection and S. suis GDH gene copies in the experimental group were highest (10(4-15)) on day 7 post infection. Data presented in this report demonstrate that the TaqMan RT-qPCR detection method can be used to characterize the dynamic changes occurring during S. suis serotype 2 blood infections in Bama minipigs thereby facilitating research associated with defining pathogenic mechanisms associated with this organism. (C) 2010 Elsevier B.V. All rights reserved.
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