Characterization of Streptococcus suis serotype 2 blood infections using RT-qPCR to quantify glutamate dehydrogenase copy numbers

文献类型: 外文期刊

第一作者: Gu, Jiangping

作者: Gu, Jiangping;Li, Caiying;Yue, Xiuwei;Yuan, Congli;Cui, Li;Hua, Xiuguo;Yang, Zhibiao;Cai, Xuehui;Liu, Yonggang;Wang, Shujie;Yang, Weijun;Hao, Yongqing;Xing, Rui;Li, Caiying;Zhang, Mingfeng

作者机构:

关键词: Streptococcus suis serotype 2;TaqMan probe;Real-time quantitative PCR;Blood infections

期刊名称:JOURNAL OF MICROBIOLOGICAL METHODS ( 影响因子:2.363; 五年影响因子:2.24 )

ISSN: 0167-7012

年卷期: 2010 年 83 卷 3 期

页码:

收录情况: SCI

摘要: This study characterized the dynamic distribution of bacteria in the blood of pigs infected with Streptococcus suis serotype 2 using specific primers and a TaqMan probe designed to amplify the highly conserved S. suis serotype 2 glutamate dehydrogenase (GDH) gene sequences. Gene copy numbers were used to determine the concentration of bacteria in the blood of infected pigs over time using established TaqMan real-time quantitative PCR methodologies (RT-qPCR). The results showed that the detection limit of the RT-qPCR was 10 GDH gene copies. The advantages of utilizing this approach are the high levels of specificity, sensitivity and reproducibility. Bacteria were detected in the blood of infected pigs after 24 h post infection and S. suis GDH gene copies in the experimental group were highest (10(4-15)) on day 7 post infection. Data presented in this report demonstrate that the TaqMan RT-qPCR detection method can be used to characterize the dynamic changes occurring during S. suis serotype 2 blood infections in Bama minipigs thereby facilitating research associated with defining pathogenic mechanisms associated with this organism. (C) 2010 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Gu, Chaojiang,Zheng, Congyi,Shi, Lili,Zhang, Qian,Li, Yong,Lu, Bin,Xiong, Yi,Qu, Sanpu,Shao, Junjun,Chang, Huiyun. 2007

[2]hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Survival Ability in Phagocytes. Xu, Bin,Zhang, Ping,Li, Weiyi,Liu, Rui,Tang, Jinsheng,Fan, Hongjie,Zhang, Ping,Fan, Hongjie. 2017

[3]Research progress in China on the virulence factors of Streptococcus suis serotype. Yue, Xiuwei,Hou, Yixuan,Hua, Xiuguo,Yang, Zhibiao,Wang, Shujie,Cai, Xuehui.

[4]Evaluation of the protective efficacy of four novel identified membrane associated proteins of Streptococcus suis serotype 2. Zhou, Yang,Wang, Yan,Deng, Limei,Zheng, Chengkun,Chen, Huanchun,Bei, Weicheng,Li, Jinquan,Yuan, Fangyan.

[5]Cloning, expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2. Yang, Wei-Jun,Yang, Zhi-Biao,Zheng, Sheng-Bo,Lan, Dao-Liang,Gu, Jiang-Ping,Xing, Rui,Yuan, Cong-Li,Cui, Li,Hua, Xiu-Guo,Cai, Xue-Hui,Yang, Wei-Jun,Hao, Yong-Qing. 2010

[6]The two-component system NisK/NisR contributes to the virulence of Streptococcus suis serotype 2. Xu, Juan,Fu, Shulin,Xu, Qiaoxia,Bei, Weicheng,Chen, Huanchun,Tan, Chen,Xu, Juan,Fu, Shulin,Xu, Qiaoxia,Bei, Weicheng,Chen, Huanchun,Tan, Chen,Liu, Manli.

[7]Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2. Zhang, Yanyan,Ding, Dandan,Yang, Xiaopei,Zong, Bingbing,Wang, Xiangru,Chen, Huanchun,Bei, Weicheng,Tan, Chen,Liu, Manli,Zhang, Yanyan,Ding, Dandan,Yang, Xiaopei,Zong, Bingbing,Wang, Xiangru,Chen, Huanchun,Bei, Weicheng,Tan, Chen,Zhang, Yanyan,Ding, Dandan,Yang, Xiaopei,Zong, Bingbing,Wang, Xiangru,Chen, Huanchun,Bei, Weicheng,Tan, Chen.

[8]Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.). Wang, Chuan Tang,Wang, Xiu Zhen,Chi, Xiao Yuan,Wu, Qi,Chen, Dian Xu,Tang, Yue Yi,Yang, Guan Pin,Feng, Tong,Gao, Hua Yuan,Xu, Ya Long. 2011

[9]Different Tolerance in Bread Wheat, Durum Wheat and Barley to Fusarium Crown Rot Disease Caused by Fusarium pseudograminearum. Liu, Yaxi,Wei, Yuming,Zheng, Youliang,Liu, Yaxi,Ma, Jun,Yan, Wei,Liu, Chunji,Ma, Jun,Yan, Guijun,Yan, Wei,Zhou, Meixue,Zhou, Meixue. 2012

[10]Analysis on Differentially Expressed Genes of Muscle Tissues in Rugao Chicken at the Ages of 2 and 12 Weeks by Microarray. Luan, De Qin,Bin Chang, Guo,Zhen, Ting,Huang, Zheng Yang,Zhou, Wei,Chen, Guo Hong,Liu, Yan. 2011

[11]Molecular identification and interaction assay of the gene (OsUbc13) encoding a ubiquitin-conjugating enzyme in rice. Wang, Ya,Xu, Meng-yun,Liu, Jian-ping,Wang, Mu-gui,Tu, Ju-min,Wang, Ya,Yin, Hai-qing. 2014

[12]Identification of a trypsin gene from Scylla paramamosain and its expression profiling during larval development. Jiang, Keji,Zhang, Fengying,Zhang, Dan,Zhang, Yong,Qiao, Zhenguo,Ma, Lingbo,Tao, Qichang,Pi, Yan. 2011

[13]Ontogeny expression of ghrelin, neuropeptide Y and cholecystokinin in blunt snout bream, Megalobrama amblycephala. Ping, H. -C.,Feng, K.,Zhang, G. -R.,Wei, K. -J.,Wang, W. -M.,Zou, G. -W.. 2014

[14]Expression Pattern Analysis of Four Mlo Genes from Rose. Qiu, Xianqin,Jian, Hongying,Wang, Qigang,Tang, Kaixue,Qiu, Xianqin,Bao, Manzhu.

[15]Large proportion of genes in one cryptic WO prophage genome are actively and sex-specifically transcribed in a fig wasp species. Wang, Guan-Hong,Xiao, Jin-Hua,Huang, Da-Wei,Wang, Guan-Hong,Niu, Li-Ming,Ma, Guang-Chang,Huang, Da-Wei. 2014

[16]Establishment of Real-time Quantitative PCR Method for Vibrio parahaemolyticus in Sediment of the Stichopus japonicus Culture Pond. Li Qiu-fen,Jiang Wei-wei,Liu Huai-de,Li Xiao-long,Jiang Wei-wei.

[17]Cloning and characterization of ubiquitin ribosome fusion gene RpS27a, a deltamethrin-resistance-associated gene from diamondback moth (Plutella xylostella L.). Zhang, Hong,Cheng, Chen,Gu, Shuyu,Zhou, Zhou,Cheng, Luogen,Li, Fengliang.

[18]In-depth analysis of the endogenous reference genes used in the quantitative PCR detection systems for rice. Zhang, Li,Cao, Yinglong,Wu, Gang,Wu, Yuhua,Lu, Changming,Liu, Xin. 2012

[19]Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea). Zhou, Ying,Li, Jing,Cheng, Jun,Zhou, Hui,Gu, Chao,Han, Yue-Peng,Guo, Dong,Gardiner, Sue. 2013

[20]A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander. Huang, Qi,Shao, Jonathan,Davis, Robert E.,Singh, Raghuwinder,Guan, Wei,Zhao, Tingchang. 2013

作者其他论文 更多>>