Identification of dispensable nucleotide sequence in 3 ' untranslated region of porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

第一作者： Sun, Zhi

作者： Sun, Zhi;Liu, Changlong;Tan, Feifei;Gao, Fei;Yuan, Shishan;Sun, Zhi;Qin, Aijian;Liu, Ping

作者机构：

关键词： Porcine reproductive and respiratory;syndrome virus;Replication Secondary structure;3 ' untranslated region (UTR)

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2010 年 154 卷 1-2 期

页码：

收录情况： SCI

摘要： The 3' untranslated region (UTR) of porcine arterivirus genome plays a pivotal role for virus replication, yet the properties of 3' UTR remain largely undefined. We conducted site-directed mutagenesis to the 3' UTR of the type II porcine reproductive and respiratory syndrome virus (PRRSV). Serial deletions of the 3' UTR showed that at least 40 nucleotides immediately following the ORF7 stop codon were dispensable for the viability of PRRSV in cultured cells. We then constructed a chimeric PRRSV cDNA clone using type II PRRSV as the backbone containing the 3' UTR from the type 1 PRRSV. The chimeric virus was viable and shared similar properties with the parental virus. Our results provided the first description of the 40nt dispensable region in type I PRRSV 3' UTR, and further predicted structure demonstrated that the high-order structure of 3' UTR might play significant roles in its function. (C) 2010 Elsevier B.V. All rights reserved.

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