Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits

文献类型： 外文期刊

第一作者： Liu, Yongfeng

作者： Liu, Yongfeng;Zan, Linsen;Zhao, Shuanping;Liu, Yongfeng;Huang, Honggang;Li, Yong;Tang, Zhonglin;Yang, Shulin;Li, Kui;Zan, Linsen

作者机构：

关键词： association analysis;body measurement;cattle;genomic structure;SNP polymorphism;Ubiquinol-cytochrome c reductase complex chaperone (UQCC)

期刊名称：MOLECULES AND CELLS （ 影响因子：5.034； 五年影响因子：4.932 ）

ISSN： 1016-8478

年卷期： 2010 年 30 卷 5 期

页码：

收录情况： SCI

摘要： Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis show that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454. Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing of an animal model using cattle to study big animal body type.

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