Identification of Fungal Strain by Fourier Transform Infrared Spectroscopy and Cluster Analysis

文献类型： 外文期刊

第一作者： Chai A Li

作者： Chai A Li;Li Jin Ping;Shi Yan Xia;Xie Xue-wen;Li Bao-ju

作者机构：

关键词： FTIR Fungi;ATR;Cluster analysis;Identification

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2010 年 30 卷 11 期

页码：

收录情况： SCI

摘要： Fourier transform attenuated total reflection infrared spectroscopy(FTIR-ATR) has been a novel technical procedure to identify and classify microorganisms in recent years In the present study Fourier transform infrared spectroscopy(FTIR) in combination with an attenuated total reflection(ATR) unit were used to discriminate important plant destroying fungi Mycelia of 17 fungal strains belonging to 14 different species were grown on potato dextrose agar(PDA)plants and subjected to FTIR ATR measurements High resolution and well reproducibility Infrared spectra were obtained, and significant spectral differences among these strains were observed in the wavenumber regions of 1 800 1 485 cm(-1) 1 485 1 185 cm(-1) and 1 185 900 cm(-1) According to the characteristic bands in these regions cluster analysis was executed to classify the FTIR spectra. The result showed that different fungal strains could be identified correctly demonstrating the high potential of FTIR ATR as a tool for fungal strain identification and classification. The method is rapid inexpensive and reproducible and requires minimum sample preparation.

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