A multiplex PCR assay for fraud identification of deer products

文献类型： 外文期刊

第一作者： Zha, Daiming

作者： Zha, Daiming;Xing, Xiumei;Yang, Fuhe;Zha, Daiming;Zha, Daiming;Xing, Xiumei;Yang, Fuhe;Zha, Daiming;Xing, Xiumei;Yang, Fuhe

作者机构：

关键词： Multiplex PCR;Species identification;Deer products;Mitochondrial genes

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2010 年 21 卷 10 期

页码：

收录情况： SCI

摘要： Attempts were made to established one-step multiplex PCR assay for the fraud identification of the mostly used species in deer products (bovine, ovine, porcine and poultry). Primers were selected from published papers or designed in the well-conserved region of tRNA-Val and 16S rRNA mitochondrial genes after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 124, 183, 225 and 290 bp length for bovine, poultry, ovine and porcine, respectively. The detection limit was 1 ng for porcine and ovine primers, 5 ng for poultry primers and 0.5 ng for bovine primers. The results demonstrated that fraud phenomena are very epidemic in the deer products, especially heart, blood, penis and antler products. The multiplex PCR described in this study, proved to be very sensitive and reliable in species identification, could be considered as a further improvement of traditional methods based assay for the identification of deer products. (C) 2010 Elsevier Ltd. All rights reserved.

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