Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

文献类型： 外文期刊

第一作者： Chen, Jing

作者： Chen, Jing;Yang, Yanping;Guo, Suxia;Peng, Jinbiao;Lin, Jiaojiao;Cheng, Guofeng;Chen, Jing;Li, Jiakui;Yang, Yanping;Liu, Zongping

作者机构：

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2010 年 107 卷 4 期

页码：

收录情况： SCI

摘要： Small non-coding RNAs including microRNAs and small interfering RNAs play important roles in many biological processes of many organisms. Argonaute proteins serve as a key component of the RNA-induced silencing complex for mediating miRNA/siRNA functions. In the present study, we systematically investigated Argonaute proteins in Schistosoma japonicum by using bioinformatics in combination with 5'- and 3'-Rapid Amplification of cDNA ends techniques and thus obtained three full-length cDNAs encoding Argonaute proteins, named as SjAgo1, SjAgo2, and SjAgo3, respectively. Additionally, SjAgo1/2/3 were differentially expressed in different developmental stages of schistosomes as determined by real-time RT-PCR and Western blot. Taken together, our preliminary results suggested that SjAgo1/2/3 may control gene expression during the life cycle of S. japonicum and therefore may regulate schistosome development and other biological processes.

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