Identification of an intergenic region that is not essential for replication of goatpox virus
文献类型： 外文期刊
第一作者： Ma, Wenge
作者： Ma, Wenge;Xue, Ying;Wang, Yan;Wei, Jie;Huang, Jiong;Fu, Zihua;Ma, Wenge;Wang, Fang;Yu, Li
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2010 年 155 卷 8 期
页码：
收录情况： SCI
摘要： A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
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