Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis

文献类型： 外文期刊

第一作者： Zhou, Jinlin

作者： Zhou, Jinlin;Liao, Min;Fujisaki, Kozo;Zhou, Jinlin;Gong, Haiyan;Xuan, Xuenan;Fujisaki, Kozo

作者机构：

关键词： Haemaphysalis longicornis;Cystatin;Hlcyst-3

期刊名称：EXPERIMENTAL AND APPLIED ACAROLOGY （ 影响因子：2.132； 五年影响因子：2.196 ）

ISSN： 0168-8162

年卷期： 2010 年 51 卷 4 期

页码：

收录情况： SCI

摘要： The gene encoding cystatin from the tick Haemaphysalis longicornis has been reported previously. In the study reported here, we characterized a member of cystatins and designated it as Hlcyst-3 (H. longicornis cystatin-3). Its full-length cDNA is 602 bp, and it encodes a putative 129 amino acid protein with an obvious signal peptide. Sequence analysis revealed that it has significant homology with the known secreted cystatin. The recombinant protein was expressed in a GST-fused soluble form in Escherichia coli and was purified by affinity chromatography. The inhibitory activity of the recombinant protein against papain and cathepsin L was identified by fluorogenic substrate analysis. Real-time PCR revealed that Hlcyst-3 was mostly expressed in the tick midgut.

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