Effects of abamectin selection on the genetic differentiation within Plutella xylostella (Lepidoptera: Plutellidae) based on amplified fragment length polymorphism

文献类型: 外文期刊

第一作者: Zhou, Xiao-Mao

作者: Zhou, Xiao-Mao;Wu, Qing-Jun;Zhang, You-Jun;Zhou, Xiao-Mao;Bai, Lian-Yang;Huang, Xiong-Ying

作者机构:

关键词: abamectin resistance;AFLP;genetic differentiation;Plutella xylostella

期刊名称:INSECT SCIENCE ( 影响因子:3.262; 五年影响因子:3.206 )

ISSN: 1672-9609

年卷期: 2010 年 17 卷 4 期

页码:

收录情况: SCI

摘要: Diamondback moth, Plutella xylostella (L.) is one of the most serious insect pests for its remarkable ability to develop resistance to virtually every insecticide that has been used against it. In the present study, amplified fragment length polymorphism (AFLP) is used to study the genetic differentiation as well as the effects of abamectin-selection on population genetic differentiation for P xylostella. A dendrogram was constructed from the matrix of genetic distances using the STATIST1CA software (Version 4.5) and unweighted pair-group method with arithmetic averages (UPGMA). The data demonstrated that compared to the susceptible strain (ABM-s), the heterozygosity in the abamectin-resistant strain (ABM-r) decreased with the increased selection pressure and resistant level. When the resistance ratio was below 4.3, there was no significant differentiation at the genome DNA level. When the resistance ratio reached 5.8, a fairly significant differentiation began to appear, and when the resistance ratio reached and exceeded 8.1, there appeared a significant genetic differentiation. The results suggest that abamectin selection is associated with increased genetic polymorphism in P xylostella.

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