Transformation of Liquidambar formosana L. via Agrobacterium tumefaciens using a mannose selection system and recovery of salt tolerant lines

文献类型： 外文期刊

第一作者： Qiao, Guirong

作者： Qiao, Guirong;Zhou, Jing;Jiang, Jing;Sun, Yuehua;Pan, Luanyin;Song, Honggai;Jiang, Jingmin;Zhuo, Renying;Sun, Yuehua;Wang, Xiaojuan;Sun, Zongxiu;Sun, Yuehua

作者机构：

关键词： Liquidambar formosana L.;Transformation;Mannose-selection marker;AtNHX1

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2010 年 102 卷 2 期

页码：

收录情况： SCI

摘要： A mannose selection system was adapted for use in the Agrobacterium-mediated transformation of Liquidambar formosana L. This system makes use of the pmi gene, which encodes phosphomannose isomerase. This protein converts mannose-6-phosphate to fructose-6-phosphate. Leaf explants from an axenic plant of L. formosana L. were infected with Agrobacterium. The gene AtNHXI was transformed into L. formosana L. using the transformation procedure developed in this study. We found that supplementing the media with 1.5% (w/v) mannose and 1% sucrose provided the necessary conditions for the selection of transformed plants from non-transformed plants. In this study, 134 positive transgenic plants were obtained. Genetic transformation was confirmed by PCR and Southern blot analysis, while RT-PCR confirmed expression of the foreign gene (AtNHX1) in the regenerated plants. Chlorophenol red assays confirmed the activity of transgenes in regenerated plants. In order to ascertain the tolerance of transformants for salt, further experimentation was done. Results showed that the soluble protein content in three transgenic lines (T-12, T-22, T-43) was higher than that in the control plant, while the MDA content was lower. Our results show that transgenic L. formosana L. plants can be obtained using either antibiotic resistance genes that are not expressed in the microorganisms or an antibiotic-free positive selection system.

分类号：

  • 相关文献

[1]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[2]Co-expression of AtNHX1 and TsVP improves the salt tolerance of transgenic cotton and increases seed cotton yield in a saline field. Cheng Cheng,Zhang, Kewei,Ying Zhang,Xiugui Chen,Jiuling Song,Zhiqiang Guo,Kunpeng Li,Kewei Zhang. 2018

[3]Overexpression of Arabidopsis thaliana Na+/H+ antiporter gene enhanced salt resistance in transgenic poplar (Populus x euramericana 'Neva'). Jiang, Chaoqiang,Zheng, Qingsong,Liu, Zhaopu,Liu, Ling,Zhao, Gengmao,Long, Xiaohua,Jiang, Chaoqiang,Xu, Wenjun. 2012

[4]Enhanced V-ATPase activity contributes to the improved salt tolerance of transgenic tobacco plants overexpressing vacuolar Na+/H+ antiporter AtNHX1. Luo, Ping,Li, Yinxin,Zhou, Shufeng,Zhang, Zhiming,Tang, Qilin,Lan, Hai. 2011

[5]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. Yang, QC,Wu, MS,Wang, PQ,Kang, JM,Zhou, XL.

[6]Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants. Shen, Guoxin,Wei, Jia,Qiu, Xiaoyun,Hu, Rongbin,Kuppu, Sundaram,Zhang, Hong,Auld, Dick,Blumwald, Eduardo,Gaxiola, Roberto,Payton, Paxton.

[7]Variant Amino Acid Residues Alter the Enzyme Activity of Peanut Type 2 Diacylglycerol Acyltransferases. Zheng, Ling,Wan, Shubo,Peng, Zhenying,Zheng, Ling,Bian, Fei,Chen, Gao,Shan, Lei,Li, Xinguo,Wan, Shubo,Peng, Zhenying,Shockey, Jay. 2017

[8]Progress in chloroplast genome analysis. Liu, Clarke Jihong,Xing Shao-Chen. 2008

[9]Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae. Rehman, Latifur,Su, Xiaofeng,Guo, Huiming,Qi, Xiliang,Cheng, Hongmei. 2016

[10]Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants. Wei, Zheng-Yi,Fan, Ming-Xia,Lin, Feng,Wei, Zheng-Yi,Zhang, Yu-Ying,Wang, Yun-Peng,Zhong, Xiao-Fang,Xing, Shao-Chen,Zhang, Yu-Ying,Xu, Nuo. 2016

[11]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[12]Transformation of sulfate and organic S in rice straw in flooded paddy soils and its availability to plants using sulfur-35 labeling. Zhou, W,Li, ST,He, P,Lin, B. 2006

[13]A new time-saving transformation system for Brassica napus. Kong, Fanming,Li, Juan,Tan, Xiaoli,Zhang, Lili,Zhang, Zhiyan,Ma, Xiaoke,Qi, Cunkou. 2009

[14]Transient beta-glucuronidase expression in asparagus protoplasts following electroporation. Chen, GY,Conner, AJ,Fautrier, AG,Field, RJ. 1999

[15]Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase. Liu, Zi-Hui,Zhang, Hong-Mei,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Yan-Min,Liu, Zi-Hui,Zhang, Hong-Mei,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Yan-Min,Chen, Shou-Yi,Liu, Gui-Bo. 2011

[16]An efficient protocol for the production of chit42 transgenic Furenzhi banana (Musa spp. AA group) resistant to Fusarium oxysporum. Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun,Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun. 2013

[17]A callus transformation system for gene functional studies in soybean. Xu Kun,Zhang Xiao-mei,Fan Cheng-ming,Chen Fu-lu,Zhu Jin-long,Fu Yong-fu,Zhang Shi-long,Chen Qing-shan. 2017

[18]Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Jones, H. D.,Li, K. X.,He, Y.,Chen, S.,Chen, X. M.,Wang, D. S.,Xia, L. Q.,Wang, D. W.. 2010

[19]Gene transfer strategy for kiwifruit to obtain pure transgenic plant through inducing adventitious roots in leaf explants with Agrobacterium tumefaciens. Li, M,Huang, ZG,Han, LX,Zhao, GR,Li, YH. 2003

[20]Research Progress on Transformation Maize Mediated by Agrobacterithm Tumefaciens. Li, Xiuping,Li, Xiuping,Jiang, Lijing,Liu, Na. 2011

作者其他论文 更多>>

微信小程序