Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.
文献类型: 外文期刊
第一作者: Zhan Gao-miao
作者: Zhan Gao-miao;Tong Jin;Wang Han-zhong;Hua Wei
作者机构:
关键词: 14-3-3;expression pattern;abiotic stress;Brassica napus L.
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2010 年 9 卷 7 期
页码:
收录情况: SCI
摘要: In eukaryotes, 14-3-3 proteins constitute a family of ubiquitous regulatory molecules. They play very important roles in many cell processes. However, their enconding genes and roles in plants remain to be elucidated. In this paper, four 14-3-3 genes from Brassica napus L. were obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 respectively. The phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2 belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon group. The transcript levels of four Bn1433 genes were analyzed in different organs, various stress conditions and some hormone treatments by real-time PCR. The result showed that all the Bn1433s were expressed constitutively in roots, stems, leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF (day after flowering). By the analysis of real-time PCR, different expression patterns of Bn1433s were studied in various stress conditions and hormone treatments. It was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genome-wide identification of stress-associated proteins (SAP) with A20/AN1 zinc finger domains associated with abiotic stresses responses in Brassica napus
作者:He Xin;Xie Sai;Xie Pan;Yao Min;Liu Wei;Qin Lunwen;Hua Wei;Liu Zhongsong;Guan Mei;Liu Zhongsong;Guan Mei;Zheng Ming;Liu Hongfang;Hua Wei
关键词:Brassica napus; SAP; A20/AN1 domain; Zinc finger; Abiotic stresses
-
A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue
作者:Hu Zhi-yong;Zhan Gao-miao;Wang Han-zhong;Hua Wei
关键词:chloroplast DNA;mitochondrial DNA;organelle isolation;rapeseed
-
Pathotypes and Genetic Diversity of Blumeria graminis f sp hordei in the Winter Barley Regions in China
作者:Zhu Jing-huan;Wang Jun-mei;Jia Qiao-jun;Yang Jian-ming;Lin Feng;Hua Wei;Shang Yi;Zhu Jing-huan;Zhou Yi-jun;Zhou Yi-jun
关键词:Blumeria graminis f sp hordei;powdery mildew;pathotype;virulence frequency;diversity;barley;China
-
Combining Ability and Genetic Effects of Germination Traits of Brassica napus L. Under Waterlogging Stress Condition
作者:Cheng Yong;Cong Ye;Zou Chong-shun;Zhang Xue-kun;Wang Han-zhong;Gu Min
关键词:Brassica napus L.;relative vigor index;waterlogging tolerance;germination trait;combining ability;diallel crossing
-
Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus
作者:Chen Bo;Wang Han-zhong;Li Yun-chang;Yan Xiao-hong;Wang Li-jun;Wei Wen-hui;Wang Tai-xia
关键词:AINTEGUMENTA gene;Brassica napus;BnaANT gene;yield-related gene;quantitative real-time PCR
-
Cloning and Sequence Analysis of Prophenoloxidase from Haemocytes of the Red Swamp Crayfish, Procambarus clarkii
作者:Li Yan-he;Chen Hong-quan;Zheng Fang-liang;Wang Han-zhong;Li Yan-he;Wang Liu-quan;Xu Di-ping
关键词:prophenoloxidase cDNA;Procambarus clarkii;nucleotide sequence;copper-binding sites;intron