Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus

文献类型： 外文期刊

第一作者： Chen Bo

作者： Chen Bo;Wang Han-zhong;Li Yun-chang;Yan Xiao-hong;Wang Li-jun;Wei Wen-hui;Wang Tai-xia

作者机构： Minist Agr, Chinese Acad Agr Sci, Inst Oil Crops, Key Lab Biol Sci Oil Crops, Wuhan 430062, Peoples R China;Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

关键词： AINTEGUMENTA gene;Brassica napus;BnaANT gene;yield-related gene;quantitative real-time PCR

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 2013影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 4 期

页码：

收录情况： SCI

摘要： AINTEGUMENTA (ANT) gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B napus cultivar Zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B napus, namely BnaX.ANT.a (GenBank accession no DQ211969) and BnaX ANT b (accession no DQ211970) They shared 87.1 and 83 4% amino acid identity with ANT of A thaliana, respectively. There is 87.4% amino acid identity between BnaX ANT a and BnaX.ANT.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B napus

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