Molecular characterization of the Puroindoline a-D1b allele and development of an STS marker in wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Chen, Feng

作者： Chen, Feng;Zhang, Fuyan;Cui, Dangqun;Morris, Craig;He, Zhonghu;Xia, Xianchun;He, Zhonghu

作者机构：

关键词： Kernel hardness;Wheat;Puroindoline

期刊名称：JOURNAL OF CEREAL SCIENCE （ 影响因子：3.616； 五年影响因子：3.891 ）

ISSN： 0733-5210

年卷期： 2010 年 52 卷 1 期

页码：

收录情况： SCI

摘要： Kernel hardness is mainly controlled by one major genetic locus on the short arm of chromosome 5D in bread wheat. Twelve Chinese and CIMMYT wheat cultivars were characterized for the deletion region of Pina-D1b genotype and developing a novel STS marker for this allele. PCR and SDS-PAGE were used to confirm the Pina-D1b genotype, and then 20 pairs of primers were designed to amplify the fragment including deletion region in Pina-D1b genotype by primer walking strategy. An STS marker Pina-N spanning deletion region in Pina-D1b was developed and sequencing results showed that all of 10 Pina-D1b genotypes uniformly possessed a 15,380 bp deletion in comparison with that of Chinese Spring with wild type. This study provided an alternative method to exam Pina-D1b by molecular marker and will accelerate identification of puroindoline alleles in bread wheat. (C) 2010 Elsevier Ltd. All rights reserved.

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