Process optimization for the enrichment of alpha-linolenic acid from silkworm pupal oil using response surface methodology

文献类型： 外文期刊

第一作者： Wang, Jun

作者： Wang, Jun;Wu, Fu-An;Liang, Yao;Wang, Meng;Wu, Fu-An

作者机构：

关键词： alpha-Linolenic acid (ALA);desilked silkworm pupal oil;urea clathration method;enrichment process optimization;response surface methodology (RSM)

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2010 年 9 卷 20 期

页码：

收录情况： SCI

摘要： alpha-Linolenic acid (ALA) was widely used in food and pharmaceutical industry, as an important medical material. In the present study, the mixed fatty acids (MFAs) extracted from desilked silkworm pupal oil were used raw materials for enriching ALA, using urea clathration method; their preparation process was optimized by response surface methodology (RSM). The research results indicated that the enrichment of ALA produced, by using urea clathration method from desilked silkworm pupal oil was effectively enhanced by RSM. Three parameters of the process for enriching ALA were optimized as follows, using RSM: the ratio of saturated urea/EtOH solution to MFAs was 2: 1(v/v); clathration temperature, 4 degrees C and clathration time, 2 h. The adequately high R(2) value, 0.9674 (adjusted R(2) = 0.9086) indicated the statistical significance of the model. The purity of ALA after optimization was 31.87%, while predicted value, 35.60%. In conclusion, enrichment conditions optimization to enhance purity of ALA from the MFAs of desilked silkworm pupal oil can be easily and effectively done by RSM. The developed enrichment process of ALA indicated desilked silkworm pupal oil as a potential raw material for producing ALA.

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