A reason for overlap peaks in direct sequencing of rRNA gene ITS in Pleurotus nebrodensis

文献类型： 外文期刊

第一作者： Huang, Chenyang

作者： Huang, Chenyang;Xu, Jingyu;Gao, Wei;Chen, Qiang;Zhang, Jinxia;Huang, Chenyang;Wang, Hexiang

作者机构：

关键词： cloning and sequencing;insertion;protoplast-derived monokaryons

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2010 年 305 卷 1 期

页码：

收录情况： SCI

摘要： To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis, we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.

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